Anti-C1D 抗体 [EPR11122] (ab166897)
![Western blot - Anti-C1D antibody [EPR11122] (ab166897)](https://www.abcam.co.jp/ps/products/166/ab166897/Images/ab166897-484039-AntiC1D-antibody-western-blot-human.jpg "Western blot - Anti-C1D antibody [EPR11122] (ab166897)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11122] to C1D
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-C1D antibody [EPR11122]
C1D 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11122] to C1D -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Human fetal heart, heart and skeletal muscle lysates. ICC/IF: MCF7 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11122 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab166897の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 16 kDa.
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| ICC/IF |
1/100 - 1/250.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 16 kDa. |
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ICC/IF
1/100 - 1/250. |
追加情報
Is unsuitable for IHC-P or IP.
ターゲット情報
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機能
Plays a role in the recruitment of the RNA exosome complex to pre-rRNA to mediate the 3'-5' end processing of the 5.8S rRNA; this function may include MPHOSPH6. Can activate PRKDC not only in the presence of linear DNA but also in the presence of supercoiled DNA. Can induce apoptosis in a p53/TP53 dependent manner. May regulate the TRAX/TSN complex formation. Potentiates transcriptional repression by NR1D1 and THRB. -
組織特異性
Ubiquitous. Expressed at very high levels in the hippocampus, medulla oblongata, mammary gland, thyroid and salivary gland. Expressed at high levels in the fetal; lung, liver and kidney. Expressed at low levels in skeletal muscle, appendix, heart, lung and colon. -
配列類似性
Belongs to the C1D family. -
翻訳後修飾
Phosphorylated by PRKDC. -
細胞内局在
Cytoplasm. Nucleus > nucleolus. EXOSC10 is required for nucleolar localization. - Information by UniProt
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参照データベース
- Entrez Gene: 10438 Human
- Entrez Gene: 57316 Mouse
- Entrez Gene: 289810 Rat
- Omim: 606997 Human
- SwissProt: Q13901 Human
- SwissProt: O35473 Mouse
- Unigene: 602900 Human
- Unigene: 697447 Human
see all -
別名
- 1110036E10Rik antibody
- AI875855 antibody
- C1D antibody
see all
画像
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Western blot - Anti-C1D antibody [EPR11122] (ab166897)All lanes : Anti-C1D antibody [EPR11122] (ab166897) at 1/1000 dilution
Lane 1 : Human heart tissue lysate with NFDM /TBST
Lane 2 : Human skeletal muscle tissue lysate with NFDM /TBST
Lane 3 : Human thyroid tissue lysate with NFDM /TBST
Lane 4 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysate with NFDM /TBST
Lane 5 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate with NFDM /TBST
Lane 6 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate with NFDM /TBST
Lane 7 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate with NFDM /TBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 1 second -
![Western blot - Anti-C1D antibody [EPR11122] (ab166897)](https://www.abcam.co.jp/ps/products/166/ab166897/Images/C1D-Primary-antibodies-ab166897-2.jpg)
Western blot - Anti-C1D antibody [EPR11122] (ab166897)All lanes : Anti-C1D antibody [EPR11122] (ab166897) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP antibody at 1/2000 dilution
Predicted band size: 16 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-C1D antibody [EPR11122] (ab166897)](https://www.abcam.co.jp/ps/products/166/ab166897/Images/C1D-Primary-antibodies-ab166897-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-C1D antibody [EPR11122] (ab166897)Immunofluorescent analysis of MCF7 cells labeling C1D using ab166897 at 1/100 dilution. -
![Anti-C1D antibody [EPR11122] (ab166897)](https://www.abcam.co.jp/ps/products/166/ab166897/Images/ab166897-3-benefits-of-recombinant-antibodies.png)
Anti-C1D antibody [EPR11122] (ab166897)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab166897 は論文での使用が確認できていません。