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リコンビナントRabMAb

Anti-C19orf2 抗体 [SP215] - BSA and Azide free (ab245758)

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Flow Cytometry (Intracellular) - Anti-C19orf2 antibody [SP215] - BSA and Azide free (ab245758)
  • Flow Cytometry (Intracellular) - Anti-C19orf2 antibody [SP215] - BSA and Azide free (ab245758)
  • Flow Cytometry (Intracellular) - Anti-C19orf2 antibody [SP215] - BSA and Azide free (ab245758)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf2 antibody [SP215] - BSA and Azide free (ab245758)
  • Anti-C19orf2 antibody [SP215] - BSA and Azide free (ab245758)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP215] to C19orf2 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-C19orf2 antibody [SP215] - BSA and Azide free
    C19orf2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [SP215] to C19orf2 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-Pmore details

  • 種交差性

    交差種: Rat, Human
    交差が予測される動物種: Mouse, Cow

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human 293 and C6 cells. Human ovarian adenocarcinoma tissue

  • 特記事項

    ab245758 is the carrier-free version of ab183310.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.20
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A/G purified

  • 特記事項（精製）

    Purified from TCS by protein A/G.

  • ポリ/モノ

    モノクローナル

  • クローン名

    SP215

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245758の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration.

Perform Antigen Retrieval by boiling tissue sections in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

 
特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration.

Perform Antigen Retrieval by boiling tissue sections in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

 

ターゲット情報

  • 機能

    Involved in gene transcription regulation. Acts as a transcriptional repressor in concert with the corepressor UXT to regulate androgen receptor (AR) transcription. May act as a tumor suppressor to repress AR-mediated gene transcription and to inhibit anchorage-independent growth in prostate cancer cells. Required for cell survival in ovarian cancer cells. Together with UXT, associates with chromatin to the NKX3-1 promoter region. Antagonizes transcriptional modulation via hepatitis B virus X protein.
    Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival sigaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity at mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination.

  • 組織特異性

    Ubiquitous. Expressed in ovarian cancers (at protein level). Expressed strongly in skeletal muscle. Expressed weakly in brain, heart, pancreas and in prostate epithelial cells.

  • 配列類似性

    Belongs to the RNA polymerase II subunit 5-mediating protein family.

  • 翻訳後修飾

    Phosphorylated. Phosphorylation occurs essentially on serine residues. Phosphorylation occurs in response to androgen treatment in prostate cancer cells in a mTOR-dependent manner. Phosphorylated; hyperhosphorylated in mitochondria in a mTORC-dependent signaling pathway. Phosphorylated at Ser-372 by RPS6KB1 in a growth factor- and rapamycin-dependent manner. S6K1-mediated mitochondrial phosphorylation at Ser-372 disrupts the URI1-PPP1CC complex in the mitochondrion, releaves PPP1CC phosphatase inhibition activity and hence engages a negative feedback diminishing RPS6KB1 kinase activity and preventing sustained S6K1-dependent signaling.

  • 細胞内局在

    Nucleus. Cytoplasm. Mitochondrion. Cell projection > dendrite. Colocalizes with PFDN2, PFDN4, PPP1CC, RPS6KB1 and STAP1 at mitochondrion.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • C19orf2 antibody
    • Chromosome 19 open reading frame 2 antibody
    • NNX3 antibody
    • NNX3 protein antibody
    • PPP1R19 antibody
    • Protein NNX3 antibody
    • Protein phosphatase 1 regulatory subunit 19 antibody
    • RMP antibody
    • RMP_HUMAN antibody
    • RNA polymerase II subunit 5 mediating protein antibody
    • RNA polymerase II subunit 5-mediating protein antibody
    • RPB5 mediating protein antibody
    • RPB5-mediating protein antibody
    • Unconventional prefoldin RPB5 interactor 1 antibody
    • Unconventional prefoldin RPB5 interactor antibody
    • URI antibody
    • URI1 antibody
    • URI1, prefoldin-like chaperone antibody
    see all

画像

  • Flow Cytometry (Intracellular) - Anti-C19orf2 antibody [SP215] - BSA and Azide free (ab245758)
    Flow Cytometry (Intracellular) - Anti-C19orf2 antibody [SP215] - BSA and Azide free (ab245758)

    Intracellular Flow Cytometry analysis of C6 (Rat glial tumor glial cell) cells labeling C19orf2 with purified ab183310 at 1/80 dilution (1.09µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183310). 

     

     

  • Flow Cytometry (Intracellular) - Anti-C19orf2 antibody [SP215] - BSA and Azide free (ab245758)
    Flow Cytometry (Intracellular) - Anti-C19orf2 antibody [SP215] - BSA and Azide free (ab245758)

    Intracellular Flow Cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling C19orf2 with purified ab183310 at 1/80 dilution (1.09µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183310). 

     

     

  • Flow Cytometry (Intracellular) - Anti-C19orf2 antibody [SP215] - BSA and Azide free (ab245758)
    Flow Cytometry (Intracellular) - Anti-C19orf2 antibody [SP215] - BSA and Azide free (ab245758)

    Intracellular flow cytometric analysis of Human 293 cells labeling C19orf2 using ab183310 at 1/100 (green), compared to a negative control rabbit IgG (blue). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183310). 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf2 antibody [SP215] - BSA and Azide free (ab245758)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C19orf2 antibody [SP215] - BSA and Azide free (ab245758)

    Immunohistochemical analysis of Human ovarian adenocarcinoma tissue labeling C19orf2 using ab183310 at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183310).

  • Anti-C19orf2 antibody [SP215] - BSA and Azide free (ab245758)
    Anti-C19orf2 antibody [SP215] - BSA and Azide free (ab245758)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab245758 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab245758 は論文での使用が確認できていません。

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