JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB122626

Anti-C12orf34 抗体

Anti-C12orf34 antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Polyclonal C12orf34 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAM222A aa 150-300.

別名を表示する

C12orf34, FAM222A, Protein FAM222A

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of cerebral cortex tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of stomach tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of testis tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-C12orf34 antibody (AB122626)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-C12orf34 antibody (AB122626)

Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli, plasma membrane, mitochondria and focal adhesions. Recommended concentration of ab122626 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Western blot - Anti-C12orf34 antibody (AB122626)
  • WB

Supplier Data

Western blot - Anti-C12orf34 antibody (AB122626)

All lanes:

Western blot - Anti-C12orf34 antibody (ab122626)

All lanes:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

Predicted band size: 47 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C12orf34 antibody (AB122626)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of skeletal muscle tissue labelling C12orf34 with ab122626 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol. No positivity in myocytes as expected.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human FAM222A aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q5U5X8

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

C12orf34 also known as chromosome 12 open reading frame 34 is a protein coding gene with a molecular mass of approximately 53 kDa. The protein expression of C12orf34 shows tissue-specific patterns prominently in the brain skeletal muscle and liver. This suggests its involvement in performing essential functions in these particular tissues. Notably it exhibits a unique sequence composition which may result in specific signaling or structural roles though precise mechanical actions remain under investigation.
Biological function summary

This protein appears to function as part of a larger protein complex indicating a collaborative role in intracellular processes. C12orf34 has been implicated in the regulation of signaling pathways that affect cellular growth and metabolism suggesting its importance in maintaining cellular homeostasis. Its activity may influence gene expression and protein synthesis connecting it to broader cellular functions within those systems where it is expressed.

Pathways

This protein acts within tightly regulated metabolic and signaling processes. It is associated with key pathways such as the PI3K-Akt pathway which is critical for regulating cell survival and growth. Through this pathway C12orf34 interacts with other proteins like mTOR a central regulator of cell metabolism. By participating in these pathways C12orf34 helps integrate external signals with cellular responses linking environmental cues with cellular growth and energy balance.

Researchers associate C12orf34 with conditions such as muscular dystrophy and neurodegenerative diseases. Its connection to these disorders suggests that mutations or dysregulation in C12orf34 or its associated pathways can disrupt normal cellular functions. Additionally the interaction of C12orf34 with proteins related to neurological health such as those in the Akt signaling pathway further supports its potential role in the onset or progression of these conditions. Understanding these links may offer insights into novel therapeutic strategies for related diseases.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

See full target information FAM222A
style
left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Translational neurodegeneration 13:24 PubMed38671492

2024

Agomirs upregulating carboxypeptidase E expression rescue hippocampal neurogenesis and memory deficits in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Dongfang Jiang,Hongmei Liu,Tingting Li,Song Zhao,Keyan Yang,Fuwen Yao,Bo Zhou,Haiping Feng,Sijia Wang,Jiaqi Shen,Jinglan Tang,Yu-Xin Zhang,Yun Wang,Caixia Guo,Tie-Shan Tang

Intractable & rare diseases research 9:217-221 PubMed33139980

2020

Reactive astrocytes express Aggregatin () in the brains of Alzheimer's disease and Nasu-Hakola disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Ichi Satoh,Yoshihiro Kino,Motoaki Yanaizu,Tsuyoshi Ishida,Yuko Saito

Nature communications 11:411 PubMed31964863

2020

FAM222A encodes a protein which accumulates in plaques in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tingxiang Yan,Jingjing Liang,Ju Gao,Luwen Wang,Hisashi Fujioka,Xiaofeng Zhu,Xinglong Wang
View all publications
style
left-column
style
left-column
style
right-column

組合せ製品

Primary Antibodies

AB177149

Anti-SFPQ antibody [EPR11847]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] (AB177149)

  • 5
  • 3
Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

  • 5
  • 104
Primary Antibodies

AB137869

Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

  • 5
  • 9
Primary Antibodies

AB214012

Anti-PSPC1 antibody [1L4] - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-PSPC1 antibody [1L4] - C-terminal (AB214012)

  • 0
  • 0

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com