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リコンビナントRabMAb

Anti-C Reactive Protein 抗体 [Y284] - Low endotoxin, Azide free (ab157242)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)
  • Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)
  • Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y284] to C Reactive Protein - Low endotoxin, Azide free
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 568

製品の概要

  • 製品名

    Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free
    C Reactive Protein 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [Y284] to C Reactive Protein - Low endotoxin, Azide free

  • 由来種

    Rabbit

  • 特異性

    This antibody recognises C Reactive Protein. This antibody is predicted to detect the splice isoform 2 as well as isoform 1.

  • アプリケーション

    適用あり: IP, WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    ab157242 is the carrier-free version of ab32412.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab157242の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

  • 組織特異性

    Found in plasma.

  • 配列類似性

    Belongs to the pentaxin family.
    Contains 1 pentaxin domain.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Pentraxin 1, short antibody
      • C reactive protein antibody
      • C reactive protein pentraxin related antibody
      • C-reactive protein(1-205) antibody
      • CRP antibody
      • CRP_HUMAN antibody
      • MGC88244 antibody
      • Pentraxin 1 antibody
      • PTX 1 antibody
      • PTX1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32412).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32412).

    • Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)
      Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)

      ab32412 (purified) at 1/30 immunoprecipitating C Reactive Protein in HepG2 whole cell lysate.

      Lane 1 (input): HepG2 whole cell lysate (10µg)

      Lane 2 (+): ab32412 + HepG2 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32412 in HepG2 whole cell lysate.

      For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32412).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human normal liver tissue labelling C Reactive Protein using unpurified ab32412 at a dilution of 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32412).

    • Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)
      Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free (ab157242)

    参考文献 (0)

    ab157242 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab157242 は論文での使用が確認できていません。

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