Anti-C Reactive Protein 抗体 [Y284] (ab32412)
![Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.co.jp/ps/products/32/ab32412/Images/ab32412-253925-anti-c-reactive-protein-antibody-y284-western-blot.jpg "Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y284] to C Reactive Protein
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-C Reactive Protein antibody [Y284]
C Reactive Protein 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [Y284] to C Reactive Protein -
由来種
Rabbit -
特異性
This antibody recognises C Reactive Protein. This antibody is predicted to detect the splice isoform 2 as well as isoform 1.
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アプリケーション
適用あり: WB, IP, IHC-Pmore details
適用なし: ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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エピトープ
ab32412 reacts with an epitope located in the C terminal region of C Reactive Protein. -
ポジティブ･コントロール
- WB: Human serum and plasma lysates. IHC-P: Normal human liver tissue. IP: HepG2 whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y284 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32412の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/2000. Predicted molecular weight: 25 kDa.
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| IP |
1/30 - 1/50.
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| IHC-P | (3) |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
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WB
1/1000 - 1/2000. Predicted molecular weight: 25 kDa. |
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IP
1/30 - 1/50. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
組織特異性
Found in plasma. -
配列類似性
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 1401 Human
- Omim: 123260 Human
- SwissProt: P02741 Human
- Unigene: 709456 Human
- Unigene: 76452 Human
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別名
- Pentraxin 1, short antibody
- C reactive protein antibody
- C reactive protein pentraxin related antibody
see all
画像
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Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)All lanes : Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/2000 dilution (purified)
Lane 1 : Human serum
Lane 2 : Human plasma
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.co.jp/ps/products/32/ab32412/Images/ab32412-253883-anti-c-reactive-protein-antibody-y284-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.co.jp/ps/products/32/ab32412/Images/ab32412-253884-anti-c-reactive-protein-antibody-y284-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.co.jp/ps/products/32/ab32412/Images/ab32412-253849-anti-c-reactive-protein-antibody-y284-immunoprecipitation.jpg)
Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412)ab32412 (purified) at 1/30 immunoprecipitating C Reactive Protein in HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate.
Lane 1 (input): HepG2 whole cell lysate (10µg)
Lane 2 (+): ab32412 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32412 in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.co.jp/ps/products/32/ab32412/Images/ab32412-44736-anti-c-reactive-protein-antibody-y284-western-blot.gif)
Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412)Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/1000 dilution (unpurified) + Human serum
Predicted band size: 25 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.co.jp/ps/products/32/ab32412/Images/ab32412-44735-anti-c-reactive-protein-antibody-y284-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (ab32412)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human normal liver tissue labelling C Reactive Protein using unpurified ab32412 at a dilution of 1/100.
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![Anti-C Reactive Protein antibody [Y284] (ab32412)](https://www.abcam.co.jp/ps/products/32/ab32412/Images/ab32412-7-benefits-of-recombinant-antibodies.png)
Anti-C Reactive Protein antibody [Y284] (ab32412)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab32412 は 36 報の論文で使用されています。
- Dumitriu D et al. Deciduous tooth biomarkers reveal atypical fetal inflammatory regulation in autism spectrum disorder. iScience 26:106247 (2023). PubMed: 36926653
- Patil PA et al. HNF-1β is a More Sensitive and Specific Marker Than C-Reactive Protein for Identifying Biliary Differentiation in Primary Hepatic Carcinomas. Arch Pathol Lab Med 146:220-226 (2022). PubMed: 34086854
- Lee JH et al. Alzheimer's disease protease-containing plasma extracellular vesicles transfer to the hippocampus via the choroid plexus. EBioMedicine 77:103903 (2022). PubMed: 35220044
- Ercan C et al. Genomic analysis of focal nodular hyperplasia with associated hepatocellular carcinoma unveils its malignant potential: a case report. Commun Med (Lond) 2:11 (2022). PubMed: 35603298
- Kim EN et al. CRP immunodeposition and proteomic analysis in abdominal aortic aneurysm. PLoS One 16:e0245361 (2021). PubMed: 34428207