Anti-c-Myc (phospho T58) 抗体 [EPR17923] (ab185655)
![Western blot - Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655)](https://www.abcam.co.jp/ps/products/185/ab185655/Images/ab185655-255239-anti-c-myc-phospho-t58-antibody-epr17923-western-blot.jpg "Western blot - Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17923] to c-Myc (phospho T58)
- Suitable for: ICC/IF, WB, Flow Cyt (Intra), Dot blot
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-c-Myc (phospho T58) antibody [EPR17923]
c-Myc 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17923] to c-Myc (phospho T58) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, Flow Cyt (Intra), Dot blotmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate, HeLa cells treated with 200nM Calyculin A and 1uM Okadaic Acid for 60 minutes whole cell lysate. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17923 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185655の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/250.
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| WB | (1) |
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 49 kDa).
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| Flow Cyt (Intra) |
1/700.
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| Dot blot |
1/1000.
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| 特記事項 |
|---|
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ICC/IF
1/250. |
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WB
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 49 kDa). |
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Flow Cyt (Intra)
1/700. |
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Dot blot
1/1000. |
ターゲット情報
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機能
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. -
関連疾患
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻訳後修飾
Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. -
細胞内局在
Nucleus > nucleoplasm. Nucleus > nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 4609 Human
- Entrez Gene: 17869 Mouse
- Entrez Gene: 24577 Rat
- Omim: 190080 Human
- SwissProt: P01106 Human
- SwissProt: P01108 Mouse
- SwissProt: P09416 Rat
- Unigene: 202453 Human
see all -
製品の状態
c-Myc is also expressed in the cytoplasm. -
別名
- AU016757 antibody
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
see all
画像
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Western blot - Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655)All lanes : Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655) at 1/5000 dilution
Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma ) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma ) treated with 200nM Calyculin A and 1uM Okadaic Acid for 60 minutes whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer was 5% NFDM /TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655)](https://www.abcam.co.jp/ps/products/185/ab185655/Images/ab185655-255250-anti-c-myc-phospho-t58-antibody-epr17923-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling c-Myc (phospho T58) with ab185655 at 1/250, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green).
Confocal image showing nuclear staining on HeLa cells. The staining decreased after blocking with phospho peptide (100μg/ml) overnight. The control peptide is a non-phospho peptide.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody -Loading Control (ab7291) at 1/1000 dilution Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/500 (red).
The negative controls are as follows:-
-ve control 1 - ab185655 at 1/500 followed by ab150120 at 1/500.
-ve control 2 -ab7291 at 1/1000 followed by ab150077 at 1/500. -
![Flow Cytometry (Intracellular) - Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655)](https://www.abcam.co.jp/ps/products/185/ab185655/Images/ab185655-7-anti-c-myc-phospho-t58-antibody-epr17923-flowcytometry-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655)Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) treated with 200nM Calyculin A and 1uM Okadaic Acid for 60 min cells labeling c-Myc with purified ab185655 at 1/700 dilution (1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Dot Blot - Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655)](https://www.abcam.co.jp/ps/products/185/ab185655/Images/ab185655-349278-AnticMyc-phospho-T58-antibody-EPR17923-dotblot-phospho-peptides.jpg)
Dot Blot - Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655)Lane 1: c-Myc (phospho T58).
Lane 2: c-Myc (pT58) non-phospho peptide.
Lane 3: c-Myc (pS62) phospho peptide.
Lane 4: c-Myc (pS62) non-phospho peptide.Dot blot analysis using ab185655 at a dilution of 1/1000. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Western blot - Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655)](https://www.abcam.co.jp/ps/products/185/ab185655/Images/ab185655-255240-anti-c-myc-phospho-t58-antibody-epr17923-western-blot.jpg)
Western blot - Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655)All lanes : Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with Lambda Phosphatase whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer was 5% NFDM /TBST.
The strong band in Lane 1 of WB-2 compared to WB-1 is due to different lysate batches and a lower dilution factor (1:1000).
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![Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655)](https://www.abcam.co.jp/ps/products/185/ab185655/Images/ab185655-6-benefits-of-recombinant-antibodies.png)
Anti-c-Myc (phospho T58) antibody [EPR17923] (ab185655)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (16)
ab185655 は 16 報の論文で使用されています。
- Ni T et al. PTBP1 drives c-Myc-dependent gastric cancer progression and stemness. Br J Cancer 128:1005-1018 (2023). PubMed: 36635500
- Sheng G et al. P2RX7 promotes osteosarcoma progression and glucose metabolism by enhancing c-Myc stabilization. J Transl Med 21:132 (2023). PubMed: 36803784
- Welcker M et al. Two diphosphorylated degrons control c-Myc degradation by the Fbw7 tumor suppressor. Sci Adv 8:eabl7872 (2022). PubMed: 35089787
- Wang D et al. A Regulatory Loop of FBXW7-MYC-PLK1 Controls Tumorigenesis of MYC-Driven Medulloblastoma. Cancers (Basel) 13:N/A (2021). PubMed: 33494392
- Li H et al. DOC-2/DAB2 interactive protein destabilizes c-Myc to impair the growth and self-renewal of colon tumor-repopulating cells. Cancer Sci 112:4593-4603 (2021). PubMed: 34449943