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リコンビナントRabMAb

Anti-c-Myb (phospho S11) 抗体 [EP769Y] (ab45150)

Reviews (1)Q&A (2)References (60)
Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
  • Dot Blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
  • Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
  • Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
  • Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
  • Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
  • Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
  • Immunoprecipitation - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
  • Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP769Y] to c-Myb (phospho S11)
  • Suitable for: WB, IHC-P, IP, Dot blot
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-c-Myb (phospho S11) antibody [EP769Y]
    c-Myb 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP769Y] to c-Myb (phospho S11)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IP, Dot blotmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Ramos, Molt-4, and HL-60 cell lysate. HeLa cell lysate untreated and treated with lambda phosphatase. IHC-P: Human cervical carcinoma tissue. IP: MOLT-4.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab45150の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/5000. Predicted molecular weight: 72 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/50.
Dot blot
1/1000.
特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 72 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/50.
Dot blot
1/1000.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 機能

    Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

  • 配列類似性

    Contains 3 HTH myb-type DNA-binding domains.

  • ドメイン

    Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression.

  • 翻訳後修飾

    Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
    Phosphorylated by NLK on multiple sites, which induces proteasomal degradation.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C myb antibody
      • c myb protein (140 AA) antibody
      • c-myb protein (140 AA) antibody
      • c-myb_CDS antibody
      • c-myb10A_CDS antibody
      • c-myb13A_CDS antibody
      • c-myb14A_CDS antibody
      • c-myb8B_CDS antibody
      • Cmyb antibody
      • efg antibody
      • Myb antibody
      • MYB proto-oncogene transcription factor antibody
      • MYB_HUMAN antibody
      • ONCOGENE AMV antibody
      • Oncogene MYB antibody
      • Proto oncogene c Myb antibody
      • Proto-oncogene c-Myb antibody
      • Transcriptional activator Myb antibody
      • v myb avian myeloblastosis viral oncogene homolog antibody
      • v-myb avian myeloblastosis viral oncogene homolog antibody
      see all

    画像

    • Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      All lanes : Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/2000 dilution (purified)

      Lane 1 : Molt-4 (Human lymphoblastic leukemia cell line) cell lysate with c-Myb (phospho S11) peptide
      Lane 2 : Molt-4 cell lysate with c-Myb (phospho S12) peptide
      Lane 3 : Molt-4 cell lysate with c-Myb (phospho S11/12) peptide
      Lane 4 : Molt-4 cell lysate with c-Myb unmodified peptide

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution

      Predicted band size: 72 kDa
      Observed band size: 77 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

      Immunohistochemical analysis of paraffin-embedded human vervical carcinoma tissue staining c-Myb with unpurified ab45150. 

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Dot Blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      Dot Blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

      Dot blot analysis of c-Myb (phospho S11/12) peptide (Lane 1) and c-Myb unmodified peptide (Lane 2) labeling c-Myb (phospho S11) with purified ab45150 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      All lanes : Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/2000 dilution (purified)

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate with c-Myc (phospho S11) peptide
      Lane 2 : HeLa cell lysate with c-Myc (phospho S12) peptide
      Lane 3 : HeLa cell lysate with c-Myc (phospho S11/12) peptide
      Lane 4 : HeLa cell lysate with c-Myc unmodified peptide

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size: 72 kDa
      Observed band size: 75,77 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      All lanes : Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/5000 dilution (purified)

      Lane 1 : Untreated Ramos (Human Burkitt's lymphoma cell line) cell lysate
      Lane 2 : Ramos cell lysate, membrane treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution

      Predicted band size: 72 kDa
      Observed band size: 75,77 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      All lanes : Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/5000 dilution (purified)

      Lane 1 : Untreated HL-60 (Human promyelocytic leukemia cell line) cell lysate
      Lane 2 : HL-60 cell lysate, membrane treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/2000 dilution

      Predicted band size: 72 kDa
      Observed band size: 77 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      All lanes : Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/2000 dilution (purified)

      Lane 1 : HeLa cell lysate, the membrane treated with lambda phosphatase
      Lane 2 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size: 72 kDa
      Observed band size: 75,77 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      Western blot - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150) at 1/10000 dilution (unpurified) + Ramos (Human Burkitt's lymphoma cell line) membrane lysate

      Predicted band size: 72 kDa
      Observed band size: 72,75 kDa why is the actual band size different from the predicted?

    • Immunoprecipitation - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      Immunoprecipitation - Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

      c-Myb was immunoprecipitated from 0.35 mg MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10 µg with ab45150 at 1/120 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10 µg

      Lane 2: ab45150 IP in MOLT-4 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab45150 in MOLT-4 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Fresh lysate should be used to minimize protein degradation. Multi-bands should be c-myb isoforms based on immunogen design and Uniprot database.

    • Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)
      Anti-c-Myb (phospho S11) antibody [EP769Y] (ab45150)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (60)

    ab45150 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab45150 は 60 報の論文で使用されています。

    • Smith C  et al. TAL1 activation in T-cell acute lymphoblastic leukemia: a novel oncogenic 3' neo-enhancer. Haematologica 108:1259-1271 (2023). PubMed: 36632736
    • Deleuze V  et al. Efficient genome editing in erythroid cells unveils novel MYB target genes and regulatory functions. iScience 26:107641 (2023). PubMed: 37670779
    • Humtsoe JO  et al. Development and Characterization of MYB-NFIB Fusion Expression in Adenoid Cystic Carcinoma. Cancers (Basel) 14:N/A (2022). PubMed: 35565392
    • Parikh AS  et al. Single-cell RNA sequencing identifies a paracrine interaction that may drive oncogenic notch signaling in human adenoid cystic carcinoma. Cell Rep 41:111743 (2022). PubMed: 36450256
    • Ji J  et al. Distinct clinicopathological and genomic features in solid and basaloid adenoid cystic carcinoma of the breast. Sci Rep 12:8504 (2022). PubMed: 35590093
    View all Publications for this product

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-v-Myb + c-Myb antibody [EP769Y] - ChIP Grade

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (chronic myelogenous leukemia & monkey kidney cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris (BioRad))
    Loading amount
    50000 cells
    Specification
    chronic myelogenous leukemia & monkey kidney cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Ragnhild Eskeland

    Verified customer

    投稿 Apr 22 2015

    Question

    I ordered ab45150, which is an antibody named Anti-v-Myb + c-Myb antibody [EP769Y] https://www.abcam.com/v-Myb-c-Myb-antibody-EP769Y-ab45150.html. I successfully blotted with c-myb protein, so the antibody is good. Now I am considering to blot with AMV-v-myb. According to the datasheet, this antibody is made against the c-terminus of c-myb. While the C-terminal homologous portion is truncated in AMV-v-myb by some literatures. Would you please check and confirm that whether this antibody will detect AMV-v-myb?

    Read More
    Answer

    Thank you for contacting us.

    This product is raised against the N-terminal region. It should not be affected by a truncated C-terminus. We do guarantee this to recognizeAvian myeloblastosis viral (v Myb) in human in the following applications; WB,IHC-P,ICC/IF,IP,ChIP.

    For UNTESTED applications, we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount equal to the value of the product tested. The terms and conditions of this offer can be found here: https://www.abcam.com/AbTrial


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    danke für Ihre schnelle Antwort.

    Ich habe verschiedene Lysate aus humanen, monozytären Zelllinien verwendet. Zum einen Ganzzellextrakte, wobei die Zellen mit Triton X 100 1% in PBS mit oder ohne Ultraschall lysiert wurden. Zum anderen habe ich Kernextrakte dieser Zellen eingesetzt, die ich mit dem Active Motif Kit generiert habe. Proteaseinhibitoren habe ich jeweils immer zugesetzt.

    Vor dem Beladen habe ich die Proben jeweils in Mercaptoethanol-haltigem Ladepuffer gekocht.

    Der von mir getestete Antikörper wurde im mm 2011 von uns bestellt- da ich einiges versucht habe und nebenbei noch andere Projekte laufen, also schon vor etwas mehr als einem halben Jahr.

    Read More
    Answer

    Vielen Dank für Ihre Antwort und für diese weiteren Informationen.

    Die Protokolldetails bestätigen meine Vermutung, dass der anti-Myb-Antikörper wohl leider fehlerhaft ist. Zwar ist die Lyse mit Ultraschall vielleicht etwas harsch und kann nach unserer Erfahrung unter Umständen zu unerwünschten Banden führen; allerdings haben Sie ja auch verschiedene Extraktionen ausprobiert, daher ist dieser Punkt sicher nicht der Knackpunkt.

    Es tut mir leid, dass ab45150nicht wie erwartet funktioniert hat. Ich weiß, dass Sie viel Zeit für diese Experimente verwendet haben und möchte Ihnen daher einen kostenlosen Ersatz oder eine Gutschrift anbieten (im Einzelfall können wir die Garantiezeit ausdehnen, und in Ihrem Fall ist diese Ausnahme absolut berechtigt!). Wenn Sie möchten, kann ich Ihnen gerne ein neues Lot von ab45150 zusenden, oder alternativ jeden anderen primären Antikörper Ihrer Wahl. Ich stelle Ihnen auch gerne eine Gutschrift aus, falls Ihnen dies im Moment besser passt.

    Um dies zu arrangieren, benötige ich die ursprüngliche Bestellung. Können Sie dazu mir folgendes bestätigen: Bestellnummer x?

    Vielen Dank für Ihre Kooperation und Hilfsbereitschaft. Ich freue mich auf Ihre Antwort.

    Read More

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