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AB109127

Anti-c-Myb 抗体 [EPR718(2)]

Anti-c-Myb antibody [EPR718(2)]

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(10 Publications)

Rabbit Recombinant Monoclonal c-Myb antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications.

別名を表示する

Transcriptional activator Myb, Proto-oncogene c-Myb, MYB

2 Images
Western blot - Anti-c-Myb antibody [EPR718(2)] (AB109127)
  • WB

Unknown

Western blot - Anti-c-Myb antibody [EPR718(2)] (AB109127)

All lanes:

Western blot - Anti-c-Myb antibody [EPR718(2)] (ab109127) at 1/1000 dilution

Lane 1:

Jurkat cell lysates at 10 µg

Lane 2:

MOLT4 cell lysates at 10 µg

Predicted band size: 72 kDa

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Western blot - Anti-c-Myb antibody [EPR718(2)] (AB109127)
  • WB

Lab

Western blot - Anti-c-Myb antibody [EPR718(2)] (AB109127)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : c-Myb knockout HAP1 whole cell lysate (20 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab109127 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab109127 was shown to specifically react with c-Myb in wild-type HAP1 cells as signal was lost in c-Myb knockout cells. Wild-type and c-Myb knockout samples were subjected to SDS-PAGE. ab109127 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-c-Myb antibody [EPR718(2)] (ab109127)

Predicted band size: 72 kDa

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関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-c-Myb antibody [EPR718(2)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR718(2)

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The c-Myb protein also known as Myb MYB or c-myb acts mechanically as a transcription factor that regulates the expression of target genes. It plays a role in cellular processes like proliferation differentiation and apoptosis by binding to specific DNA sequences. c-Myb has a molecular mass of roughly 75 kDa. It predominantly expresses in hematopoietic cells where it significantly influences gene transcription activity. Also it appears in some epithelial tissues indicating its versatile role in different cell types.
Biological function summary

The protein serves as a master regulator in the development of various cell types. It is not part of a traditional complex but interacts with other proteins to exert its function such as binding with co-activators or repressors to modulate gene expression. c-Myb controls the transcription of genes that are critical for cell cycle regulation and differentiation especially in the context of blood cell lineages. The protein is particularly influential in the production of red blood cells and white blood cells.

Pathways

C-Myb fits integrally into known hematopoietic signaling cascades and the Wnt signaling pathway. It works alongside other proteins like cyclin-dependent kinase (CDK) family members and transcription factor GATA-1 to modulate lineage-specific gene expression. Through its regulatory actions c-Myb influences the activation of downstream effectors that propagate signals essential for maintaining cellular functions within these pathways. The modulation of these pathways underlines its role in cell fate decisions.

Studies link c-Myb to several forms of leukemia and colon cancer. In leukemia aberrant expression of c-Myb disrupts normal hematopoiesis contributing to the uncontrolled proliferation of immature blood cells. The abnormal interactions between c-Myb and proteins like Bcl-2 can lead to enhanced cellular survival further promoting malignant conditions. In colon cancer c-Myb overexpression influences tumor growth and progression often acting alongside other oncogenic factors to enhance disease severity.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
See full target information MYB
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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

British journal of cancer 126:1205-1214 PubMed34837075

2021

MYB interacts with androgen receptor, sustains its ligand-independent activation and promotes castration resistance in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sanjeev Kumar Srivastava,Mohammad Aslam Khan,Shashi Anand,Haseeb Zubair,Sachin Kumar Deshmukh,Girijesh Kumar Patel,Seema Singh,Joel Andrews,Bin Wang,James Elliot Carter,Ajay Pratap Singh

Frontiers in oncology 11:761693 PubMed34868977

2021

The Clinical Significance and Transcription Regulation of a DNA Damage Repair Gene, SMC4, in Low-Grade Glioma Integrated Bioinformatic Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Zhisheng Wu

Cell death & disease 11:832 PubMed33028809

2020

LncRNA LL22NC03-N14H11.1 promoted hepatocellular carcinoma progression through activating MAPK pathway to induce mitochondrial fission.

Applications

Unspecified application

Species

Unspecified reactive species

Tingzhuang Yi,Hongcheng Luo,Fengxue Qin,Qi Jiang,Shougao He,Tonghua Wang,Jianwei Su,Sien Song,Xiaoshan Qin,Yueqiu Qin,Xihan Zhou,Zansong Huang

Blood cells, molecules & diseases 69:1-9 PubMed29227829

2017

A long noncoding RNA from the HBS1L-MYB intergenic region on chr6q23 regulates human fetal hemoglobin expression.

Applications

Unspecified application

Species

Unspecified reactive species

Tasha A Morrison,Ibifiri Wilcox,Hong-Yuan Luo,John J Farrell,Ryo Kurita,Yukio Nakamura,George J Murphy,Shuaiying Cui,Martin H Steinberg,David H K Chui

Leukemia 32:882-889 PubMed29089643

2017

Targeting acute myeloid leukemia by drug-induced c-MYB degradation.

Applications

Unspecified application

Species

Unspecified reactive species

V Walf-Vorderwülbecke,K Pearce,T Brooks,M Hubank,M M van den Heuvel-Eibrink,C M Zwaan,S Adams,D Edwards,J Bartram,S Samarasinghe,P Ancliff,A Khwaja,N Goulden,G Williams,J de Boer,O Williams

Oncotarget 8:30138-30150 PubMed28404913

2017

A novel role of CKIP-1 in promoting megakaryocytic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiao Fan,Yiwu Wang,Yuan Shen,Qingyan Liu,Rong Gao,Ya Qiu,Chanjuan Wang,Lingqiang Zhang

Science signaling 7:ra47 PubMed24847116

2014

Targeting poly(ADP-ribose) polymerase and the c-Myb-regulated DNA damage response pathway in castration-resistant prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Likun Li,Wenjun Chang,Guang Yang,Chengzhen Ren,Sanghee Park,Theodoros Karantanos,Styliani Karanika,Jianxiang Wang,Jianhua Yin,Parantu K Shah,Hirayama Takahiro,Masato Dobashi,Wenling Zhang,Eleni Efstathiou,Sankar N Maity,Ana M Aparicio,Elsa M Li Ning Tapia,Patricia Troncoso,Bradley Broom,Lianchun Xiao,Hyun-Sung Lee,Ju-Seog Lee,Paul G Corn,Nora Navone,Timothy C Thompson

Oncogene 34:436-44 PubMed24469049

2014

TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

A R Cyr,M V Kulak,J M Park,M V Bogachek,P M Spanheimer,G W Woodfield,L S White-Baer,Y Q O'Malley,S L Sugg,A K Olivier,W Zhang,F E Domann,R J Weigel

Blood cells, molecules & diseases 52:68-75 PubMed23910958

2013

GFI1B, EVI5, MYB--additional genes that cooperate with the human BCL6 gene to promote the development of lymphomas.

Applications

IHC

Species

Unspecified reactive species

Beverly W Baron,John Anastasi,Juraj Bies,Poluru L Reddy,Loren Joseph,Michael J Thirman,Kristen Wroblewski,Linda Wolff,Joseph M Baron

Endocrine-related cancer 19:485-96 PubMed22569827

2012

Oestrogen-induced genes in ductal carcinoma in situ: their comparison with invasive ductal carcinoma.

Applications

IHC-P

Species

Human

Akiko Ebata,Takashi Suzuki,Kiyoshi Takagi,Yasuhiro Miki,Yoshiaki Onodera,Yasuhiro Nakamura,Fumiyoshi Fujishima,Kazuyuki Ishida,Mika Watanabe,Kentaro Tamaki,Takanori Ishida,Noriaki Ohuchi,Hironobu Sasano
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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