Anti-c-Myb 抗体 [ANA236B] (ab169111)
![Western blot - Anti-c-Myb antibody [ANA236B] (ab169111)](https://www.abcam.co.jp/ps/products/169/ab169111/Images/ab169111-297399-anti-c-myb-antibody-ana236b-western-blot.jpg "Western blot - Anti-c-Myb antibody [ANA236B] (ab169111)")
Key features and details
- Rat monoclonal [ANA236B] to c-Myb
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
製品の概要
-
製品名
Anti-c-Myb antibody [ANA236B]
c-Myb 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [ANA236B] to c-Myb -
由来種
Rat -
アプリケーション
適用あり: WBmore details
適用なし: ICC,IHC-Fr,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow
-
免疫原
Recombinant fragment (His-tag) corresponding to Human c-Myb aa 402-640.
Sequence:FLNTSSNHE NSDLEMPSLT STPLIGHKLT VTTPFHRDQT VKTQKENTVF RTPAIKRSIL ESSPRTPTPF KHALAAQEIK YGPLKMLPQT PSHLVEDLQD VIKQESDESG IVAEFQENGP PLLKKIKQEV ESPTDKSGNF FCSHHWEGDS LNTQLFTQTS PVADAPNILT SSVLMAPASE DEDNVLKAFT VPKNRSLASP LQPCSSTWEP ASCGKMEEQM TSSSQARKYV NAFSARTLVM
Database link: P10242 -
ポジティブ･コントロール
- Recombinant Human c-Myb protein (ab114406) can be used as a positive control in WB. Hek-V5-c-Myb transfected cells; KARPAS299, Jurkat, MT2 and SUP-T1 cell lysates; Human thymus, bone marrow and lymphoblastic lymphoma tissues.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
ポリ/モノ
モノクローナル -
クローン名
ANA236B -
アイソタイプ
IgG2a -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab169111の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/50. Predicted molecular weight: 72 kDa.
|
| 特記事項 |
|---|
|
WB
1/50. Predicted molecular weight: 72 kDa. |
ターゲット情報
-
機能
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. -
配列類似性
Contains 3 HTH myb-type DNA-binding domains. -
ドメイン
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression. -
翻訳後修飾
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 317776 Cow
- Entrez Gene: 4602 Human
- Entrez Gene: 17863 Mouse
- Omim: 189990 Human
- SwissProt: P46200 Cow
- SwissProt: P10242 Human
- SwissProt: P06876 Mouse
- Unigene: 606320 Human
see all -
別名
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
see all
画像
-
Western blot - Anti-c-Myb antibody [ANA236B] (ab169111)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: c-Myb knockout HAP1 whole cell lysate (20 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab169111 observed at 75 kDa. Red - loading control, ab181602, observed at 37 kDa.
Ab169111 was shown to specifically react with c-Myb, in wild type HAP1 cells as signal was lost in c-Myb knockout cells. Wild-type and c-Myb knockout samples were subjected SDS-PAGE. Ab169111 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/50 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) preabsorbed and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-c-Myb antibody [ANA236B] (ab169111)](https://www.abcam.co.jp/ps/products/169/ab169111/Images/ab169111-170983-anti-c-myb-antibody-ana236b-western-blot.jpg)
Western blot - Anti-c-Myb antibody [ANA236B] (ab169111)All lanes : Anti-c-Myb antibody [ANA236B] (ab169111) at 1/50 dilution
Lane 1 : DAUDI cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : Raji cell lysate
Lane 4 : KARPAS299 cell lysate
Lane 5 : HUT78 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : MT2 cell lysate
Lane 8 : SUP-T1 cell lysate
Lane 9 : YT cell lysate
Lysates/proteins at 150 µg per lane.
Predicted band size: 72 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab169111 は論文での使用が確認できていません。