Anti-c-Myb 抗体 (ab177510)
Key features and details
- Rabbit polyclonal to c-Myb
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-c-Myb antibody
c-Myb 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to c-Myb -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human c-Myb aa 175-225. The exact sequence is proprietary. (NP_005366.2)
Sequence:GRTDNA IKNHWNSTMR RKVEQEGYLQ ESSKASQPAV ATSFQKNSHL MGFAQ
Database link: P10242 -
ポジティブ･コントロール
- Human breast carcinoma tissue; MOLT4 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8. -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab177510 was affinity purified using an epitope specific to c-Myb immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177510の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. -
配列類似性
Contains 3 HTH myb-type DNA-binding domains. -
ドメイン
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression. -
翻訳後修飾
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 317776 Cow
- Entrez Gene: 102137614 Cynomolgus monkey
- Entrez Gene: 101136017 Gorilla
- Entrez Gene: 4602 Human
- Entrez Gene: 100452954 Orangutan
- Entrez Gene: 712321 Rhesus monkey
- Omim: 189990 Human
- SwissProt: P46200 Cow
see all -
別名
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
see all
画像
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Immunoprecipitation - Anti-c-Myb antibody (ab177510)
Detection of Human c-Myb by Western Blot of Immunoprecipitate.
Lane 1: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MOLT4 cells.
Lane 2: Control IgG.
ab177510 used for IP at 6 μg/mg lysate.
ab177510 was used at 1 μg/ml for WB.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab177510)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling c-Myb with ab177510 at 1µg/ml. Detection:DAB.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab177510 は 1 報の論文で使用されています。
- Godfrey L et al. DOT1L inhibition reveals a distinct subset of enhancers dependent on H3K79 methylation. Nat Commun 10:2803 (2019). PubMed: 31243293