Anti-c-Jun (phospho T91 + T93) 抗体 [C-J 4C4] (ab13671)
Key features and details
- Mouse monoclonal [C-J 4C4] to c-Jun (phospho T91 + T93)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4]
c-Jun 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [C-J 4C4] to c-Jun (phospho T91 + T93) -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human c-Jun (phospho T91 + T93).
-
ポジティブ・コントロール
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
-
精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
C-J 4C4 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13671の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use a concentration of 5 µg/ml.
|
|
ICC/IF |
Use a concentration of 1 µg/ml.
|
特記事項 |
---|
IHC-P
Use a concentration of 5 µg/ml. |
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
-
機能
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). -
配列類似性
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. -
翻訳後修飾
Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 3725 Human
- Omim: 165160 Human
- SwissProt: P05412 Human
- Unigene: 696684 Human
-
別名
- Activator protein 1 antibody
- AP 1 antibody
- AP-1 antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4] (ab13671)ICC/IF image of ab13617 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13617, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4] (ab13671)
IHC image of c-Jun (phospho T91 + T93) staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13671, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (6)
ab13671 は 6 報の論文で使用されています。
- Hu G et al. MicroRNA-145 attenuates TNF-a-driven cartilage matrix degradation in osteoarthritis via direct suppression of MKK4. Cell Death Dis 8:e3140 (2017). IHC-P ; Human . PubMed: 29072705
- Kudo K et al. Inhibition of Gli1 results in altered c-Jun activation, inhibition of cisplatin-induced upregulation of ERCC1, XPD and XRCC1, and inhibition of platinum-DNA adduct repair. Oncogene 31:4718-24 (2012). Human . PubMed: 22266871
- Madeo A et al. c-Jun activation is required for 4-hydroxytamoxifen-induced cell death in breast cancer cells. Oncogene 29:978-91 (2010). WB ; Human . PubMed: 19935718
- Vinciguerra M et al. Negative charged threonine 95 of c-Jun is essential for c-Jun N-terminal kinase-dependent phosphorylation of threonine 91/93 and stress-induced c-Jun biological activity. Int J Biochem Cell Biol 40:307-16 (2008). PubMed: 17920329
- Hartz AM et al. Diesel exhaust particles induce oxidative stress, proinflammatory signaling, and P-glycoprotein up-regulation at the blood-brain barrier. FASEB J : (2008). PubMed: 18474546
- Nateri AS et al. The ubiquitin ligase SCFFbw7 antagonizes apoptotic JNK signaling. Science 303:1374-8 (2004). PubMed: 14739463