Anti-c-Jun (phospho S73) 抗体 (ab30620)
.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho S73) antibody (ab30620)")
Key features and details
- Rabbit polyclonal to c-Jun (phospho S73)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-c-Jun (phospho S73) antibody
c-Jun 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to c-Jun (phospho S73) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human c-Jun (phospho S73).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide.The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Corresponding non-phospho antibody
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab30620の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 36 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 36 kDa. |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). -
配列類似性
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. -
翻訳後修飾
Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3725 Human
- Omim: 165160 Human
- SwissProt: P05412 Human
- Unigene: 696684 Human
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別名
- Activator protein 1 antibody
- AP 1 antibody
- AP-1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho S73) antibody (ab30620)
Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue sections, using 1/50 Phospho c-Jun(Ser73) Antibody (ab30620). Left: untreated sample. Right: sample preincubated with synthesized phosphopeptide.
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.jpg)
Western blot - Anti-c-Jun (phospho S73) antibody (ab30620)All lanes : Anti-c-Jun (phospho S73) antibody (ab30620) at 1/500 dilution
Lane 1 : extracts from untreated Hela cells
Lane 2 : extracts from UV treated Hela cells
Predicted band size: 36 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab30620 は 18 報の論文で使用されています。
- Jing L et al. Targeting the up-regulated CNOT3 reverses therapeutic resistance and metastatic progression of EGFR-mutant non-small cell lung cancer. Cell Death Discov 9:406 (2023). PubMed: 37919290
- Xu X et al. YAP-TEAD up-regulates IRS2 expression to induce and deteriorate oesophageal cancer. J Cell Mol Med 25:2584-2595 (2021). PubMed: 33570213
- Ma Y et al. Ror2-mediated non-canonical Wnt signaling regulates Cdc42 and cell proliferation during tooth root development. Development 148:N/A (2021). PubMed: 33323370
- Gilman KE et al. Indomethacin Treatment Post-irradiation Improves Mouse Parotid Salivary Gland Function via Modulation of Prostaglandin E2 Signaling. Front Bioeng Biotechnol 9:697671 (2021). PubMed: 34381764
- Gao W et al. Psoralen inhibits the inflammatory response and mucus production in allergic rhinitis by inhibiting the activator protein 1 pathway and the downstream expression of cystatin‑SN. Mol Med Rep 24:N/A (2021). PubMed: 34278468