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リコンビナントRabMAb

Anti-c-Jun (phospho S63) 抗体 [Y172] - BSA and Azide free (ab227533)

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Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
  • Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
  • Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
  • Immunocytochemistry/ Immunofluorescence - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
  • ELISA - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
  • Dot Blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
  • Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y172] to c-Jun (phospho S63) - BSA and Azide free
  • Suitable for: WB, IHC-P, Dot blot, ICC/IF, ELISA
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 647 Alexa Fluor® 750 Unconjugated

製品の概要

  • 製品名

    Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free
    c-Jun 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [Y172] to c-Jun (phospho S63) - BSA and Azide free

  • 由来種

    Rabbit

  • 特異性

    The antibody only detects c-Jun phosphorylated on Serine 63 when tested in WB and ICC using specific phospho-treatments. However, in DotBlot and ELISA assays we detected some cross-reactivity with the non-phospho peptide as well. Please refer to the images on the datasheet. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • アプリケーション

    適用あり: WB, IHC-P, Dot blot, ICC/IF, ELISAmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: UV or Anisomycin treated NIH/3T3 or HeLa whole cell lysate (ab150035). IHC-P: Human breast carcinoma tissue. ICC/IF: A431 cells.

  • 特記事項

    ab227533 is the carrier-free version of ab32385.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab227533の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 36 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
Dot blot
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 36 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
Dot blot
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).

  • 配列類似性

    Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.

  • 翻訳後修飾

    Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Activator protein 1 antibody
    • AP 1 antibody
    • AP-1 antibody
    • AP1 antibody
    • cJun antibody
    • Enhancer Binding Protein AP1 antibody
    • Jun Activation Domain Binding Protein antibody
    • JUN antibody
    • Jun oncogene antibody
    • JUN protein antibody
    • Jun proto oncogene antibody
    • JUN_HUMAN antibody
    • JUNC antibody
    • Oncogene JUN antibody
    • p39 antibody
    • Proto oncogene c jun antibody
    • Proto oncogene cJun antibody
    • Proto-oncogene c-jun antibody
    • Transcription Factor AP 1 antibody
    • Transcription factor AP-1 antibody
    • Transcription Factor AP1 antibody
    • V jun avian sarcoma virus 17 oncogene homolog antibody
    • V jun sarcoma virus 17 oncogene homolog (avian) antibody
    • V jun sarcoma virus 17 oncogene homolog antibody
    • V-jun avian sarcoma virus 17 oncogene homolog antibody
    • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
    see all

画像

  • Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
    Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
    All lanes : Anti-c-Jun (phospho S63) antibody [Y172] (ab32385) at 0.1 µg/ml (purfied)

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with 250 ng/ml anisomycin for 30 minutes whole cell lysates
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) treated with 250 ng/ml anisomycin for 30 minutes whole cell lysates. Then the membrane was incubated with phosphatase

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32385).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling c-Jun with Purified ab32385 at 1:250 dilution (0.46 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32385).

  • Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
    Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
    All lanes : Anti-c-Jun (phospho S63) antibody [Y172] (ab32385) at 0.1 µg/ml (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1 ug/ml anisomycin for 15 minutes whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1 ug/ml anisomycin for 15 minutes whole cell lysates 15ug. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

     

  • Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
    Western blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
    All lanes : Anti-c-Jun (phospho S63) antibody [Y172] (ab32385) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1ug/mL anisomycin for 15 minutes whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1ug/ml anisomycin for 15 minutes whole cell lysates. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32385).

  • Immunocytochemistry/ Immunofluorescence - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
    Immunocytochemistry/ Immunofluorescence - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling c-Jun (phospho S63) with ab32385 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing the expression was increased after treatment with anisomycin (1 µg/ml for 15 minutes), then decreased after treatment with the Lambda Protein Phosphatase treatment 31? for 2 hours. The nuclear counter stain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32385).

  • ELISA - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
    ELISA - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)

    Antigen pS63:c-Jun (phospho S63); NP:c-Jun non-phospho. Antigen concentration 0.01~1 ng/ml.

    Primary antibody concentration range 0~1000 ng/ml.

    Secondary antibody is an Alkaline Phosphatase-conjugated Goat Anti-Rabbit IgG(H+L) used at a 1:2500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32385).

  • Dot Blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
    Dot Blot - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)

    Unpurified ab32385 used at a 1:1000 dilution.
    Secondary antibody is Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) used at a 1:100,000 dilution. Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
    Lane 1: Human c-Jun (pS63) phospho peptide.
    Lane 2: Human c-Jun non-phospho peptide.
    Exposure time 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32385).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)

    This IHC data was generated using the same anti-c Jun antibody clone, Y172, in a different buffer formulation (cat# ab32385).

    Ab32385, at a 1/50 dilution, staining c-Jun in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.

  • Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)
    Anti-c-Jun (phospho S63) antibody [Y172] - BSA and Azide free (ab227533)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (38)

ab227533 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab227533 は 38 報の論文で使用されています。

  • Wang G  et al. Exenatide exerts a neuroprotective effect against diabetic cognitive impairment in rats by inhibiting apoptosis: Role of the JNK/c-JUN signaling pathway. Mol Med Rep 25:N/A (2022). PubMed: 35119079
  • Lakkappa N  et al. Soluble epoxide hydrolase inhibitor, APAU, protects dopaminergic neurons against rotenone induced neurotoxicity: Implications for Parkinson's disease. Neurotoxicology 70:135-145 (2019). PubMed: 30472438
  • Li X  et al. Hepatic loss of Lissencephaly 1 (Lis1) induces fatty liver and accelerates liver tumorigenesis in mice. J Biol Chem 293:5160-5171 (2018). PubMed: 29475944
  • Liu S  et al. Sinomenine inhibits lipopolysaccharide-induced inflammatory injury by regulation of miR-101/MKP-1/JNK pathway in keratinocyte cells. Biomed Pharmacother 101:422-429 (2018). WB ; Human . PubMed: 29501764
  • Niu RN  et al. Overexpression of Egr2 and Egr4 protects rat brains against ischemic stroke by downregulating JNK signaling pathway. Biochimie 149:62-70 (2018). WB ; Rat . PubMed: 29580816
View all Publications for this product

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