Anti-c-Fos 抗体 [N486/32] - BSA and Azide free (ab302668)

This data was developed using ab302667, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

The band(s) beneath the target band are likely to be degraded target fragments (PMID: 9737957 and PMID: 20498278).

Exposure time:

Lanes 1-2: 26 seconds
Lanes 3-4: 81 seconds
Lanes 5-6: 136 seconds. 

This data was developed using ab302667, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cenrvical cancer tissue labeling c-Fos with AB302667 at 1/500 dilution (1.676 μg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human cervical cancer was observed. The section was incubated with ab302667 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody (610-4141) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. 

Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins was used. 

This data was developed using ab302667, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling c-Fos with AB302667 at 1/500 dilution (1.676 μg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human colon was observed. The section was incubated with ab302667 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody (610-4141) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. 

Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins was used. 

This data was developed using ab302667, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling c-Fos with AB302667 at 1/500 dilution (1.676 μg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse cerebrum was observed. The section was incubated with ab302667 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody (610-4141) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. 

Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins was used. 

This data was developed using ab302667, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling c-Fos with AB302667 at 1/500 dilution (1.676 μg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat cerebrum was observed. The section was incubated with ab302667 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody (610-4141) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. 

Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins was used. 

This data was developed using ab302667, the same antibody clone in a different buffer formulation.

Immunocytochemical/Immunofluorescent analysis of 4% paraformaldehyde fixed and 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labeling c-Fos with ab302667 at 1:250 dilution (3.4 μg/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1:1000 (2 μg/mL). Confocal image showing increased nuclear staining in HeLa cells after starvation 16 hours, then treated with TPA (200 nM) for 4 h. 

ab179513 Anti-beta Tubulin rabbit monoclonal antibody (Red) was used as a counterstain Tubulin at 1:500 dilution (4 μg/ml). Nuclear counter satin is DAPI (Blue). 

This data was developed using ab302667, the same antibody clone in a different buffer formulation.

Immunocytochemical/Immunofluorescent analysis of 4% paraformaldehyde fixed and 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) labeling c-Fos with ab302667 at 1:250 dilution (3.4 μg/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1:1000 (2 μg/mL). Confocal image showing increased nuclear staining in NIH/3T3 cells after starvation for 16 hours, then treated with TPA (200 nM) and MG-132(10 μM) for 4 h. 

Recombinant Alexa Fluor^® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab202272) (Red) was used as a counterstain Tubulin at 1:50 dilution (10 μg/ml). Nuclear counter satin is DAPI. Nuclear counterstained with DAPI (Blue).