Anti-c-Fos 抗体 [N486/32] (ab302667)

Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

The band(s) beneath the target band are likely to be degraded target fragments (PMID: 9737957 and PMID: 20498278). 

Exposure time:

Lanes 1-2: 26 seconds
Lanes 3-4: 81 seconds
Lanes 5-6: 136 seconds.

Immunohistochemical analysis of paraffin-embedded human cenrvical cancer tissue labeling c-Fos with AB302667 at 1/500 dilution (1.676 μg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human cervical cancer was observed. The section was incubated with ab302667 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody (610-4141) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. 

Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins was used. 

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling c-Fos with AB302667 at 1/500 dilution (1.676 μg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human colon was observed. The section was incubated with ab302667 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody (610-4141) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. 

Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins was used. 

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling c-Fos with AB302667 at 1/500 dilution (1.676 μg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse cerebrum was observed. The section was incubated with ab302667 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody (610-4141) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. 

Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins was used. 

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling c-Fos with AB302667 at 1/500 dilution (1.676 μg/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat cerebrum was observed. The section was incubated with ab302667 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody (610-4141) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. 

Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). 

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins was used. 

Immunocytochemical/Immunofluorescent analysis of 4% paraformaldehyde fixed and 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labeling c-Fos with ab302667 at 1:250 dilution (3.4 μg/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1:1000 (2 μg/mL). Confocal image showing increased nuclear staining in HeLa cells after starvation 16 hours, then treated with TPA (200 nM) for 4 h. 

ab179513 Anti-beta Tubulin rabbit monoclonal antibody (Red) was used as a counterstain Tubulin at 1:500 dilution (4 μg/ml). Nuclear counter satin is DAPI (Blue). 

Immunocytochemical/Immunofluorescent analysis of 4% paraformaldehyde fixed and 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) labeling c-Fos with ab302667 at 1:250 dilution (3.4 μg/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1:1000 (2 μg/mL). Confocal image showing increased nuclear staining in NIH/3T3 cells after starvation for 16 hours, then treated with TPA (200 nM) and MG-132(10 μM) for 4 h. 

Recombinant Alexa Fluor^® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab202272) (Red) was used as a counterstain Tubulin at 1:50 dilution (10 μg/ml). Nuclear counter satin is DAPI. Nuclear counterstained with DAPI (Blue).