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リコンビナントRabMAb

Anti-c-Fos 抗体 [EPR24046-20] - BSA and Azide free (ab289726)

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Western blot - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)
  • Immunoprecipitation - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)
  • Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24046-20] to c-Fos - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Rat

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Unconjugated

製品の概要

  • 製品名

    Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free
    c-Fos 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24046-20] to c-Fos - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, IHC-P, WBmore details
    適用なし: Flow Cyt or IHC-Fr

  • 種交差性

    交差種: Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: PC-12 starved overnight, then treated with 100ng/ml NGF and 10uM MG-132 for 2 hours, whole cell lysate IHC-P: Rat cerebrum, hypothalamus and hippocampus tissue. IP: PC-12 starved overnight, then treated with 100ng/ml NGF and 10uM MG-132 for 2 hours, whole cell lysate

  • 特記事項

    ab289726 is the carrier free version of ab289723.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289726の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 55-62 kDa (predicted molecular weight: 41 kDa).
特記事項
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 55-62 kDa (predicted molecular weight: 41 kDa).
追加情報
Is unsuitable for Flow Cyt or IHC-Fr.

ターゲット情報

  • 機能

    Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.

  • 配列類似性

    Belongs to the bZIP family. Fos subfamily.
    Contains 1 bZIP domain.

  • 翻訳後修飾

    Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
    Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Activator protein 1 antibody
      • AP 1 antibody
      • C FOS antibody
      • Cellular oncogene c fos antibody
      • Cellular oncogene fos antibody
      • FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody
      • FBJ murine osteosarcoma viral oncogene homolog antibody
      • FBJ murine osteosarcoma viral v fos oncogene homolog antibody
      • FBJ Osteosarcoma Virus antibody
      • FOS antibody
      • FOS protein antibody
      • FOS_HUMAN antibody
      • G0 G1 switch regulatory protein 7 antibody
      • G0/G1 switch regulatory protein 7 antibody
      • G0S7 antibody
      • Oncogene FOS antibody
      • p55 antibody
      • proto oncogene c Fos antibody
      • Proto oncogene protein c fos antibody
      • Proto-oncogene c-Fos antibody
      • v fos FBJ murine osteosarcoma viral oncogene homolog antibody
      see all

    画像

    • Western blot - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)
      Western blot - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)
      All lanes : Anti-c-Fos antibody [EPR24046-20] (ab289723) at 1/1000 dilution

      Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) starved overnight, whole cell lysate
      Lane 2 : PC-12 starved overnight, then treated with 100ng/ml NGF and 10uM MG-132 for 2 hours, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 41 kDa
      Observed band size: 55-62 kDa why is the actual band size different from the predicted?


      Exposure time: 92 seconds


      This data was developed using ab289723, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)

      This data was generated with ab289723, the same antibody in a different formulation.

      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling c-Fos at 1/100 (5.46μg/ml) for 30 mins at room temperature, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Nuclear staining on rat cerebrum. The nuclear counterstain is Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)

      This data was generated with ab289723, the same antibody in a different formulation.

      Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labelling c-Fos at 1/100 (5.46μg/ml) for 30 mins at room temperature, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Nuclear staining on rat hippocampus. The nuclear counterstain is Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)

      This data was generated with ab289723, the same antibody in a different formulation.

      Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labelling c-Fos at 1/100 (5.46μg/ml) for 30 mins at room temperature, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Nuclear staining on rat hypothalamus. The nuclear counterstain is Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunoprecipitation - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)
      Immunoprecipitation - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)

      This data was generated with ab289723, the same antibody in a different formulation.

      c-Fos was immunoprecipitated from PC-12 whole cell lysate with ab289723 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab289723 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366), was used as secondary antibody at 1/5000 dilution.

      Lane 1: PC-12 starved overnight, then treated with 100ng/ml NGF and 10uM MG-132 for 2 hours whole cell lysate 10 μg (Input).

      Lane 2: ab289723 IP PC-12 starved overnight, then treated with 100ng/ml NGF and 10uM MG-132 for 2 hours whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289723 in PC-12 starved overnight, then treated with 100ng/ml NGF and 10uM MG-132 for 2 hours whole cell lysate.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 8 seconds.

    • Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)
      Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (ab289726)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab289726 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab289726 は論文での使用が確認できていません。

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