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リコンビナントRabMAb

Anti-BTNL3 抗体 [EPR8411(2)(B)] (ab177473)

Submit a review Submit a question References (1)
Flow Cytometry (Intracellular) - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)
  • Western blot - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)
  • Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8411(2)(B)] to BTNL3
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-BTNL3 antibody [EPR8411(2)(B)]
    BTNL3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR8411(2)(B)] to BTNL3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details
    適用なし: ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human BTNL3 aa 300-400. The exact sequence is proprietary.
    Database link: Q6UXE8

  • ポジティブ･コントロール

    • Human small intestine, Jurkat, Caco2 and K562 lysates. K562 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR8411(2)(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab177473の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.

ターゲット情報

  • 組織特異性

    Expressed in small intestine, colon, testis, spleen, and leukocyte.

  • 配列類似性

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 B30.2/SPRY domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • BTNL3 antibody
      • BTNL3_HUMAN antibody
      • BTNLR antibody
      • Butyrophilin like protein 3 antibody
      • Butyrophilin like receptor antibody
      • Butyrophilin-like protein 3 antibody
      • Butyrophilin-like receptor antibody
      • COLF4100 antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)
      Flow Cytometry (Intracellular) - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)

      Intracellular flow cytometric analysis of permeabilized K562 cells labeling BTNL3with ab177473 at 1/10 dilution (red) or a rabbit IgG (negative) (green). 

       

       

    • Western blot - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)
      Western blot - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)
      All lanes : Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473) at 1/1000 dilution

      Lane 1 : Human small intestine lysate
      Lane 2 : Jurkat lysate
      Lane 3 : Caco2 lysate
      Lane 4 : K562 lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 52 kDa

    • Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)
      Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473)

    参考文献 (1)

    ab177473 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab177473 は 1 報の論文で使用されています。

    • Ramello MC  et al. An immunoproteomic approach to characterize the CAR interactome and signalosome. Sci Signal 12:N/A (2019). PubMed: 30755478

    レビューと Q&A

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