Anti-BTK 抗体 (ab25971)
Key features and details
- Rabbit polyclonal to BTK
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-BTK antibody
BTK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BTK -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to 16 N terminal amino acids near the amino terminus of Human BTK.
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ポジティブ・コントロール
- U937 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
BTK Antibody is affinity chromatography purified via peptide column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25971の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 73 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 73 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes. -
関連疾患
Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD). -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻訳後修飾
Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein. -
細胞内局在
Cytoplasm. Membrane. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 695 Human
- Omim: 300300 Human
- SwissProt: Q06187 Human
- Unigene: 159494 Human
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別名
- Agammaglobulinaemia tyrosine kinase antibody
- AGMX 1 antibody
- AGMX1 antibody
see all
画像
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Lane 1 : Anti-BTK antibody (ab25971) at 0.5 µg/ml
Lane 2 : Anti-BTK antibody (ab25971) at 1 µg/ml
Lane 3 : Anti-BTK antibody (ab25971) at 2 µg/ml
All lanes : for analysis of U937 cell lysate (15ug lysate/lane)
Predicted band size: 73 kDa -
ab52971 at 10µg/ml staining BTK in Daudi cells by ICC/IF
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Immunofluorescence of BTK in Daudi cells using ab25971 at 10 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab25971 は論文での使用が確認できていません。