Anti-BTG1 抗体 [EPR8274(2)] (ab151740)
![Western blot - Anti-BTG1 antibody [EPR8274(2)] (ab151740)](https://www.abcam.co.jp/ps/products/151/ab151740/Images/ab151740-158834-anti-btg1-antibody-epr82742-western-blot.jpg "Western blot - Anti-BTG1 antibody [EPR8274(2)] (ab151740)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8274(2)] to BTG1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-BTG1 antibody [EPR8274(2)]
BTG1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8274(2)] to BTG1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Human fetal kidney, SH-SY5Y, NIH/3T3, and MCF-7 cell lysates. Flow Cyt (intra): SH-SY5Y cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR8274(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151740の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
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| 特記事項 |
|---|
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 19 kDa. |
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.
ターゲット情報
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関連性
BTG1 belongs to the BTG family of proteins.FUNCTION: Anti-proliferative protein. Its expression is associated with the early G1 phase of the cell cycle. A chromosomal aberration involving BTG1 may be a cause of a form of B cell chronic lymphocytic leukemia; translocation t(8;12)(q24;q22) with MYC. -
参照データベース
- Entrez Gene: 694 Human
- Entrez Gene: 12226 Mouse
- Entrez Gene: 29618 Rat
- Omim: 109580 Human
- SwissProt: P62324 Human
- SwissProt: P62325 Mouse
- SwissProt: Q63073 Rat
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別名
- B cell translocation gene 1 protein antibody
- B cell translocation gene 1, anti proliferative antibody
- BTG1 protein antibody
- Protein BTG1 antibody
画像
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Western blot - Anti-BTG1 antibody [EPR8274(2)] (ab151740)All lanes : Anti-BTG1 antibody [EPR8274(2)] (ab151740) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : NIH/3T3 cell lysate
Lane 4 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 19 kDa -
![Flow Cytometry (Intracellular) - Anti-BTG1 antibody [EPR8274(2)] (ab151740)](https://www.abcam.co.jp/ps/products/151/ab151740/Images/ab151740-158833-anti-btg1-antibody-epr82742-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-BTG1 antibody [EPR8274(2)] (ab151740)Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells labeling BTG1 with ab151740 antibody at 1/10 dilution (Red), or with a rabbit IgG (Green, negative).
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![Anti-BTG1 antibody [EPR8274(2)] (ab151740)](https://www.abcam.co.jp/ps/products/151/ab151740/Images/ab151740-3-benefits-of-recombinant-antibodies.png)
Anti-BTG1 antibody [EPR8274(2)] (ab151740)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab151740 は 8 報の論文で使用されています。
- Wang D & Yan C MicroRNA-208a-3p participates in coronary heart disease by regulating the growth of hVSMCs by targeting BTG1. Exp Ther Med 23:71 (2022). PubMed: 34934442
- Deng J et al. Autophagy inhibition prevents glucocorticoid-increased adiposity via suppressing BAT whitening. Autophagy 16:451-465 (2020). PubMed: 31184563
- Al-Wabli RI et al. New Isatin-Indole Conjugates: Synthesis, Characterization, and a Plausible Mechanism of Their in vitro Antiproliferative Activity. Drug Des Devel Ther 14:483-495 (2020). PubMed: 32099332
- Zhu J & Han S Lidocaine inhibits cervical cancer cell proliferation and induces cell apoptosis by modulating the lncRNA-MEG3/miR-421/BTG1 pathway. Am J Transl Res 11:5404-5416 (2019). PubMed: 31632519
- Qian Y et al. The treatment effects and the underlying mechanism of B cell translocation gene 1 on the oncogenesis of brain glioma. J Cell Biochem N/A:N/A (2019). PubMed: 30916818
- Li E et al. microRNA-27a-3p Down-regulation Inhibits Malignant Biological Behaviors of Ovarian Cancer by Targeting BTG1. Open Med (Wars) 14:577-585 (2019). PubMed: 31410369
- Lee AS et al. eIF3 targets cell-proliferation messenger RNAs for translational activation or repression. Nature 522:111-4 (2015). WB . PubMed: 25849773
- Sun GG et al. The expression of BTG1 is downregulated in nasopharyngeal carcinoma and possibly associated with tumour metastasis. Mol Biol Rep 41:5979-88 (2014). Human . PubMed: 24985971