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AB259761

Anti-BTC 抗体 [EPR22344-85] - BSA and Azide free (Capture)

Anti-BTC antibody [EPR22344-85] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal BTC antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

別名を表示する

Bcn, Btc, Probetacellulin

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Sandwich ELISA - Anti-BTC antibody [EPR22344-85] - BSA and Azide free (Capture) (AB259761)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-BTC antibody [EPR22344-85] - BSA and Azide free (Capture) (AB259761)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab255722).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR22344-85

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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製品の詳細

ab259761 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Betacellulin. The recommended pair for sandwich ELISA is:
Capture: ab259761, Mouse Betacellulin (BTC) Capture Antibody (unconjugated)
Detector: ab259762, Mouse Betacellulin (BTC) Detector Antibody (unconjugated)
The reference range value is 4.69 - 300 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The BTC protein also known as betacellulin is a member of the epidermal growth factor (EGF) family that plays an important role in cell signaling. It is a growth factor with a molecular weight of approximately 32 kDa. BTC is expressed in a range of tissues including the pancreas kidney and small intestine. This protein binds to and activates the EGF receptor (EGFR) stimulating various cellular processes. Researchers often use the BTC mouse and BTC chimeras to study its function and physiological roles due to their relevance to human biology.
Biological function summary

BTC plays an important role in regulating cell proliferation especially in the pancreas where it influences beta-cell growth and regeneration. It operates as part of a signaling complex that interacts with other members of the EGF family impacting various cellular responses. Researchers employ experimental tools like BTC ELISA kits to quantify BTC levels in biological samples aiding in the exploration of its biological functions and mechanisms. BTC’s ability to modulate growth pathways makes it a significant molecule under investigation.

Pathways

BTC participates in the EGF/EGFR signaling pathway which is essential for controlling cellular growth and differentiation. This pathway involves the interaction of BTC with other ligands such as EGF and transforming growth factor-alpha (TGF-α) altering downstream signaling events. BTC further contributes to the activation of the MAPK/ERK pathway a critical process for transmitting signals from the cell surface to the DNA in the nucleus. Through these pathways BTC impacts cellular activities and balances multiple biological functions.

BTC is linked to conditions such as diabetes and various cancers. In diabetes BTC is associated with pancreatic function particularly influencing insulin production through its role in beta-cell regulation. In cancer abnormal BTC expression or its interactions with EGFR signaling components can lead to uncontrolled cell proliferation. Researchers study BTC’s interactions with proteins like EGF and TGF-α to understand its involvement in these disorders as therapeutic targeting of this axis holds potential for treatment strategies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
See full target information Btc
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組合せ製品

Assay Kits

AB255722

Mouse Betacellulin ELISA Kit (BTC)

Recombinant|SimpleStep

Sandwich ELISA - Mouse Betacellulin ELISA Kit (BTC) (AB255722)

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Primary Antibodies

AB6046

Anti-beta Tubulin antibody - Loading Control

BOND RX™ Validated

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Primary Antibodies

AB214672

Anti-c-Fos antibody [EPR20769]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-c-Fos antibody [EPR20769] (AB214672)

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  • 4
Primary Antibodies

AB179513

Anti-beta Tubulin antibody [EPR16774] - Loading Control

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-beta Tubulin antibody [EPR16774] - Loading Control (AB179513)

  • 5
  • 2
Primary Antibodies

AB244641

Anti-BAFF antibody [EPR18573-171] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-BAFF antibody [EPR18573-171] - BSA and Azide free (Capture) (AB244641)

  • 0
  • 0

Abcam product promise

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