Anti-BRSK1 抗体 (ab115475)
Key features and details
- Rabbit polyclonal to BRSK1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-BRSK1 antibody
BRSK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BRSK1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human BRSK1 (C terminal).
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ポジティブ･コントロール
- Human brain cortex tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115475の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 87 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 87 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Required for the polarization of forebrain neurons which endows axons and dendrites with distinct properties, possibly by locally regulating phosphorylation of microtubule-associated proteins (By similarity). May be involved in the regulation of G2/M arrest in response to UV- or methyl methane sulfonate (MMS)-induced, but not IR-induced, DNA damage. Phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo. -
組織特異性
Widely expressed, with highest levels in brain and testis. Protein levels remain constant throughout the cell cycle. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
細胞内局在
Cytoplasm. Nucleus. Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS-induced DNA damage. - Information by UniProt
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参照データベース
- Entrez Gene: 84446 Human
- Entrez Gene: 381979 Mouse
- Entrez Gene: 499073 Rat
- Omim: 609235 Human
- SwissProt: Q8TDC3 Human
- SwissProt: Q5RJI5 Mouse
- SwissProt: B2DD29 Rat
- Unigene: 182081 Human
see all -
別名
- BR serine/threonine kinase 1 antibody
- BR serine/threonine protein kinase 1 antibody
- BR serine/threonine-protein kinase 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRSK1 antibody (ab115475)ab115475, at 5µg/ml, staining BRSK1 in formalin-fixed, paraffin-embedded Human brain cortex tissue by immunohistochemistry using a biotinylated anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Western blot - Anti-BRSK1 antibody (ab115475)Lane 1 : Anti-BRSK1 antibody (ab115475) at 0.5 µg/ml
Lane 2 : Anti-BRSK1 antibody (ab115475) at 1 µg/ml
All lanes : Human brain tissue lysate
Predicted band size: 87 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab115475 は論文での使用が確認できていません。