Anti-BRN3B / POU4F2 抗体 (ab166945)
Key features and details
- Goat polyclonal to BRN3B / POU4F2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-BRN3B / POU4F2 antibody -
製品の詳細
Goat polyclonal to BRN3B / POU4F2 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Zebrafish, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- WB: Human cerebellum, mouse and rat brain tissue lysate.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab166945 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab166945の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml.
Primary incubation 1 hour at room temperature is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Primary incubation 1 hour at room temperature is recommended for this product. |
ターゲット情報
-
機能
Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons. -
組織特異性
Brain. Seems to be specific to the retina. -
配列類似性
Belongs to the POU transcription factor family. Class-4 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
細胞内局在
Nucleus speckle. - Information by UniProt
-
参照データベース
- Entrez Gene: 101132525 Chicken
- Entrez Gene: 428735 Chicken
- Entrez Gene: 471317 Chimpanzee
- Entrez Gene: 532453 Cow
- Entrez Gene: 482637 Dog
- Entrez Gene: 5458 Human
- Entrez Gene: 18997 Mouse
- Entrez Gene: 171355 Rat
see all -
別名
- Brain specific homeobox/POU domain protein 3B antibody
- Brain-3B antibody
- Brain-specific homeobox/POU domain protein 3B antibody
see all
画像
-
Western blot - Anti-BRN3B / POU4F2 antibody (ab166945)All lanes : Anti-BRN3B / POU4F2 antibody (ab166945) at 1 µg/ml
Lane 1 : Human Cerebellum tissue lysate
Lane 2 : Human Cerebellum tissue lysate with Immunizing peptide
Lane 3 : Mouse Brain tissue lysate
Lane 4 : Mouse Brain tissue lysate with Immunizing peptide
Lane 5 : Rat Brain tissue lysate
Lane 6 : Rat Brain tissue lysate with Immunizing peptide
Lysates/proteins at 35 µg per lane.
Observed band size: 35 kDa why is the actual band size different from the predicted?
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab166945 は論文での使用が確認できていません。
