AB233391

Anti-Brk/PTK6 抗体 [EPR21051-68]

Anti-Brk/PTK6 antibody [EPR21051-68]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal Brk/PTK6 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

別名を表示する

BRK, PTK6, Protein-tyrosine kinase 6, Breast tumor kinase, Tyrosine-protein kinase BRK

3 Images

Flow Cytometry (Intracellular) - Anti-Brk/PTK6 antibody [EPR21051-68] (AB233391)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Brk/PTK6 antibody [EPR21051-68] (AB233391)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized T-47D (human ductal breast epithelial tumor cell line) cell line labeling Brk/PTK6 with ab233391 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-Brk/PTK6 antibody [EPR21051-68] (AB233391)

Brk/PTK6 was immunoprecipitated from 0.35 mg of T-47D (human ductal breast epithelial tumor cell line) whole cell lysate with ab233391 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233391 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : T-47D whole cell lysate 10 μg (Input).

Lane 2 : ab233391 IP in T-47D whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab233391 in T-47D whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Brk/PTK6 antibody [EPR21051-68] (ab233391)

Predicted band size: 52 kDa

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Supplier Data

Western blot - Anti-Brk/PTK6 antibody [EPR21051-68] (AB233391)

Exposure time : Lanes 1-3 : 3 minutes; Lane 4 : 75 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Brk/PTK6 antibody [EPR21051-68] (ab233391) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

Human breast cancer lysate at 20 µg

Lane 4:

T-47D (human ductal breast epithelial tumor cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 52 kDa

Observed band size: 48 kDa

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR21051-68

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt (Intra), IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/60", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Brk known also as PTK6 is a non-receptor tyrosine kinase. Its molecular mass is approximately 52 kDa. PTK6 is expressed in various tissues with higher levels found in epithelial tissues such as breast skin and gastrointestinal tissues. Mechanically PTK6 phosphorylates different substrates affecting their function and participating in signal transduction. It acts intracellularly influencing cellular processes like proliferation differentiation and migration.

Biological function summary

PTK6 plays a role in cellular signaling systems influencing growth and survival. It does not belong to a large protein complex but often interacts with several downstream proteins to exert its effects. PTK6 modulates pathways in normal epithelial tissue development and homeostasis. In addition it regulates functions like apoptosis and motility significant for tissue maintenance and repair.

Pathways

PTK6 relates to key signaling cascades such as the MAPK and PI3K-Akt pathways. Within these pathways PTK6 is often associated with proteins like ERK1/2 and Akt which are important for cell survival and growth regulation. PTK6 can activate or inhibit these pathways influencing the cellular response to various stimuli and sometimes altering processes like cell cycle progression.

PTK6 has been implicated in the development and progression of certain cancers such as breast and prostate cancer. Elevated PTK6 expression levels have been linked to poor prognosis in these cancers where it appears to enhance tumor cell proliferation and invasion. The kinase interacts with proteins like HER2 and EGFR which are also involved in these cancers potentially affecting therapeutic responses and resistance to treatments.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins : KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors : STAT3 and STAT5A/B and a variety of signaling molecules : ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Phosphorylates the GTPase-activating protein ARAP1 following EGF stimulation which enhances EGFR signaling by delaying EGFR down-regulation (PubMed : 20554524). Also associates with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways.. Isoform 2. Inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins.

See full target information PTK6

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 18:126 PubMed40495228

2025

MACS and acellular autologous non-ovarian tissue scaffolds: a promising strategy for safe and efficient follicle transplantation in hematologic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lijun Yin,Yating Zhao,Peiwen Zhang,Tongyun Qi,Peilin Han,Luya Cai,Jun Pan,Yongyi Yang,Jie Shi,Shi Feng,Yinying Zou,Kangxin He,Guoliang Xie,Weihua He,Xinhui Zhou,Jianhua Qian

Frontiers in pharmacology 13:868296 PubMed35401239

2022

FSCN1 Promotes Esophageal Carcinoma Progression Through Downregulating PTK6 its RNA-Binding Protein Effect.

Applications

Unspecified application

Species

Unspecified reactive species

Hongfei Cai,Rui Wang,Ze Tang,Tianyu Lu,Youbin Cui

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Brk/PTK6 抗体 [EPR21051-68]

Anti-Brk/PTK6 antibody [EPR21051-68]

Flow Cytometry (Intracellular) - Anti-Brk/PTK6 antibody [EPR21051-68] (AB233391)

Immunoprecipitation - Anti-Brk/PTK6 antibody [EPR21051-68] (AB233391)

Western blot - Anti-Brk/PTK6 antibody [EPR21051-68] (AB233391)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise