Anti-BRF2 抗体 (ab17011)
Key features and details
- Goat polyclonal to BRF2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-BRF2 antibody
BRF2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to BRF2 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide: QAARQAATSVPNPP, corresponding to C terminal amino acids 406-419 of Human BRF2.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17011の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. -
配列類似性
Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 55290 Human
- Omim: 607013 Human
- SwissProt: Q9HAW0 Human
- Unigene: 726335 Human
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別名
- B-related factor 2 antibody
- BRF-2 antibody
- BRF2 antibody
see all
画像
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ab17011 at 1.5
µ g/ml detecting BRF2 from HeLa cell lysate (35ug total protein per lane) by Western blot. Primary antibody was incubated for 1 hour. The blot was detected by chemiluminescence.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab17011 は 4 報の論文で使用されています。
- Wang J et al. Transcription factor AP-2α activates RNA polymerase III-directed transcription and tumor cell proliferation by controlling expression of c-MYC and p53. J Biol Chem 299:102945 (2023). PubMed: 36707053
- Gouge J et al. Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2. Cell 163:1375-87 (2015). PubMed: 26638071
- Lockwood WW et al. Integrative genomic analyses identify BRF2 as a novel lineage-specific oncogene in lung squamous cell carcinoma. PLoS Med 7:e1000315 (2010). WB ; Human . PubMed: 20668658
- Oler AJ et al. Human RNA polymerase III transcriptomes and relationships to Pol II promoter chromatin and enhancer-binding factors. Nat Struct Mol Biol 17:620-8 (2010). IP ; Human . PubMed: 20418882