Anti-BrdU 抗体 (ab152095)
Key features and details
- Rabbit polyclonal to BrdU
- Suitable for: ICC/IF, Flow Cyt, IHC-P
- Reacts with: Species independent
- Isotype: IgG
製品の概要
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製品名
Anti-BrdU antibody
BrdU 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BrdU -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, Flow Cyt, IHC-Pmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to BrdU.
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ポジティブ･コントロール
- ICC/IF: HeLa cells treated with 100M BrdU for 4hrs. IHC-P: mouse BrdU treated intestine Flow Cyt: HeLa treated with 10 M BrdU for 30 minutes
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab152095の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100 - 1/1000.
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| Flow Cyt |
1/50 - 1/200.
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| IHC-P | (1) |
1/100 - 1/1000.
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| 特記事項 |
|---|
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ICC/IF
1/100 - 1/1000. |
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Flow Cyt
1/50 - 1/200. |
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IHC-P
1/100 - 1/1000. |
ターゲット情報
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関連性
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. -
細胞内局在
Nuclear -
別名
- Bromodeoxyuridine antibody
- BUdr antibody
画像
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Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)
HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BrdU stained by BrdU antibody (ab152095) diluted at 1/2000. Blue: Hoechst 33342 staining.
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Flow Cytometry - Anti-BrdU antibody (ab152095)ab152095 detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4ºC for 15 minutes by flow cytometry analysis. Black: Unlabelled sample was used as a control. Red: BrdU antibody (ab152095) dilution: 1/50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody (ab152095)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse BrdU treated intestine using ab152095 diluted at 1/500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min -
Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody (ab152095)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells treated with or without 100µM BrdU for 4 hours, labeling BrdU with ab152095 at 1/50 dilution. Lower images stained with Hoechst 33342.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (62)
ab152095 は 62 報の論文で使用されています。
- Yang JL et al. Transient neurogenesis in ischemic cortex from Sox2+ astrocytes. Neural Regen Res 18:1521-1526 (2023). PubMed: 36571357
- Garlapati C et al. PLK1 and AURKB phosphorylate survivin differentially to affect proliferation in racially distinct triple-negative breast cancer. Cell Death Dis 14:12 (2023). PubMed: 36627281
- Soleymanjahi S et al. RBM47 regulates intestinal injury and tumorigenesis by modifying proliferation, oxidative response, and inflammatory pathways. JCI Insight 8:N/A (2023). PubMed: 37014710
- Williamson MR et al. Subventricular zone cytogenesis provides trophic support for neural repair in a mouse model of stroke. Nat Commun 14:6341 (2023). PubMed: 37816732
- Huang L et al. Paneth cell-derived iNOS is required to maintain homeostasis in the intestinal stem cell niche. J Transl Med 21:852 (2023). PubMed: 38007452