Anti-Brd4 抗体 (ab84776)
Key features and details
- Rabbit polyclonal to Brd4
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Brd4 antibody
Brd4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Brd4 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to a region between residue 1312 and 1362 of human (NP_490597.1).
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特記事項
Concentration is optimized for IHC and not determined
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab84776 was affinity purified using an epitope specific to BRD4 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84776の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/500.
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| 特記事項 |
|---|
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IHC-P
1/100 - 1/500. |
ターゲット情報
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機能
Plays a role in a process governing chromosomal dynamics during mitosis. -
組織特異性
Ubiquitously expressed. -
関連疾患
Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein. -
配列類似性
Contains 2 bromo domains. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 484869 Dog
- Entrez Gene: 23476 Human
- Entrez Gene: 57261 Mouse
- Entrez Gene: 100533540 Pig
- Entrez Gene: 362844 Rat
- Omim: 608749 Human
- SwissProt: O60885 Human
- SwissProt: Q9ESU6 Mouse
see all -
別名
- Brd4 antibody
- BRD4-NUT FUSION antibody
- BRD4-NUT fusion oncoprotein antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody (ab84776)Staining of Brd4 by Immunohistochemistry on a formalin fixed, paraffin embedded section of human colon carcinoma using a 1/500 dilution of ab84776. Detection: DAB staining
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody (ab84776)Staining of Brd4 by Immunohistochemistry on a formalin fixed, paraffin embedded section of mouse teratoma using a 1/100 dilution of ab84776. Detection: DAB staining
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab84776 は 5 報の論文で使用されています。
- Zhu X et al. The BET PROTAC inhibitor dBET6 protects against retinal degeneration and inhibits the cGAS-STING in response to light damage. J Neuroinflammation 20:119 (2023). PubMed: 37217935
- Shahid S et al. Small Molecule BRD4 Inhibitors Apabetalone and JQ1 Rescues Endothelial Cells Dysfunction, Protects Monolayer Integrity and Reduces Midkine Expression. Molecules 27:N/A (2022). PubMed: 36364277
- Sacta MA et al. Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages. Elife 7:N/A (2018). PubMed: 29424686
- Bansal K et al. The transcriptional regulator Aire binds to and activates super-enhancers. Nat Immunol 18:263-273 (2017). PubMed: 28135252
- Yoshida H et al. Brd4 bridges the transcriptional regulators, Aire and P-TEFb, to promote elongation of peripheral-tissue antigen transcripts in thymic stromal cells. Proc Natl Acad Sci U S A 112:E4448-57 (2015). WB . PubMed: 26216992