Name: Anti-BRD1/BRL antibody [EPR12960]
Brand: RabMAb
SKU: ab181060
Rating: 5 (2 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12960] to BRD1/BRL
Suitable for: WB, IHC-P, IP
Reacts with: Mouse, Rat, Human

Carrier Free

製品名

Anti-BRD1/BRL antibody [EPR12960]
BRD1/BRL 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR12960] to BRD1/BRL
由来種

Rabbit
アプリケーション

適用あり: WB, IHC-P, IPmore details
適用なし: ICC/IF
種交差性

交差種: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: K-562 and Raji cell lysate; IHC-P: Human testis and lung carcinoma tissue, Mouse testis tissue and Rat testis tissue. IP: K-562 whole cell lysate, Mouse testis tissue lysate
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR12960
アイソタイプ

IgG
研究分野
- Epigenetics and Nuclear Signaling
- Bromodomains

Alternative Versions
- Anti-BRD1/BRL antibody [EPR12960] - BSA and Azide free (ab250317)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Raji whole cell lysate (ab30124)
- K-562 whole cell lysate (ab7911)
Recombinant Protein
- Recombinant Human BRD1/BRL protein (ab187427)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab181060の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(1)	1/1000 - 1/2000. Predicted molecular weight: 120 kDa.
IHC-P		1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP		1/30.

特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 120 kDa.
IHC-P 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.

追加情報

Is unsuitable for ICC/IF.

機能

Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity.
組織特異性

Highly expressed in testis.
配列類似性

Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain.
細胞内局在

Nucleus.
Target information above from: UniProt accession O95696 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 23774 Human
- Entrez Gene: 223770 Mouse
- Entrez Gene: 315210 Rat
- Omim: 604589 Human
- SwissProt: O95696 Human
- Unigene: 127950 Human
別名
- BR140-like protein antibody
- BRD1 antibody
- BRD1_HUMAN antibody
- BRL antibody
- Bromodomain and PHD finger-containing protein 2 antibody
- Bromodomain containing 1 antibody
- Bromodomain-containing protein 1 antibody
- BRPF2 antibody
- DKFZp686F0325 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat testis (PMID: 10602503). The section was incubated with ab181060 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Western blot - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

All lanes : Anti-BRD1/BRL antibody [EPR12960] (ab181060) at 1/2000 dilution

Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 120 kDa

Blocking/ Dilution buffer: 5% NFDM /TBST.
Immunoprecipitation - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

ab181060 at 1/30 dilution (2μg in 0.35mg lysates) immunoprecipitating BRD1/BRL in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate.

Lane 1 (input): K-562 whole cell lysate (10µg)

Lane 2 (+): ab181060 + K-562 whole cell lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181060 in K-562 whole cell lysate

For western blotting, ab181060 at 1/1000 dilution (0.691 μg/mL) followed by VeriBlot for IP (HRP) (ab131366) was used for detection (1/5000).

Blocking and Diluting buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis (PMID: 10602503). The section was incubated with ab181060 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunoprecipitation - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

ab181060 at 1/30 dilution (2μg in 0.35mg lysates) immunoprecipitating BRD1/BRL in mouse testis tissue lysate.

Lane 1 (input): Mouse testis tissue lysate (10µg)

Lane 2 (+): ab181060 + Mouse testis tissue lysate

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181060 in mouse testis tissue lysate

For western blotting, ab181060 at 1/1000 dilution (0.691 μg/mL) followed by VeriBlot for IP (HRP) (ab131366) was used for detection (1/5000).

Blocking and Diluting buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human lung carcinoma. The section was incubated with ab181060 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling BRD1/BRL with ab181060 at 1/200 dilution (3.46 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human testis (PMID: 10602503). The section was incubated with ab181060 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Anti-BRD1/BRL antibody [EPR12960] (ab181060)

Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab181060 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab181060 は 2 報の論文で使用されています。

Li Z et al. Expression Characteristics and Clinical Correlations of BRD1 in Colorectal Cancer Samples. Technol Cancer Res Treat 20:15330338211039678 (2021). PubMed: 34482774
Kulej K et al. Time-resolved Global and Chromatin Proteomics during Herpes Simplex Virus Type 1 (HSV-1) Infection. Mol Cell Proteomics 16:S92-S107 (2017). PubMed: 28179408

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-2 of 2 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Breast and Endometrial cell lines)

Gel Running Conditions

Reduced Denaturing (4-12% bis tris)

Loading amount

3 µg

Treatment

siCON or si-BRD1 smartpool for 24 hours

Specification

Breast and Endometrial cell lines

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Katie Lynch

Verified customer

投稿 Dec 31 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Ovary)

Permeabilization

Yes - 0.5% Triton

Specification

Ovary

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Katie Lynch

Verified customer

投稿 Dec 29 2021

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (2)

レビューと Q&A

Western blot abreview for Anti-BRD1/BRL antibody [EPR12960]

Immunocytochemistry/ Immunofluorescence abreview for Anti-BRD1/BRL antibody [EPR12960]