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リコンビナントRabMAb

Anti-BRAF35 抗体 [EPR9915] (ab167415)

Submit a review Submit a question References (1)
Western blot - Anti-BRAF35 antibody [EPR9915] (ab167415)
  • Immunocytochemistry/ Immunofluorescence - Anti-BRAF35 antibody [EPR9915] (ab167415)
  • Anti-BRAF35 antibody [EPR9915] (ab167415)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9915] to BRAF35
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-BRAF35 antibody [EPR9915]
    BRAF35 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR9915] to BRAF35

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details
    適用なし: Flow Cyt,IHC-P or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Jurkat, A431 and HeLa cell lysates. A431 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR9915

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab167415の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
ICC/IF
1/100 - 1/250.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
ICC/IF
1/100 - 1/250.
追加情報
Is unsuitable for Flow Cyt,IHC-P or IP.

ターゲット情報

  • 機能

    Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.

  • 組織特異性

    Ubiquitously expressed in adult tissues.

  • 配列類似性

    Contains 1 HMG box DNA-binding domain.

  • 細胞内局在

    Nucleus. Chromosome. Localized to condensed chromosomes in mitosis in conjunction with BRCA2.
  • Information by UniProt

  • 参照データベース

    • 別名

      • BRAF25 antibody
      • BRAF35 antibody
      • BRCA2 associated factor 35 antibody
      • BRCA2-associated factor 35 antibody
      • High Mobility Group Protein 20B antibody
      • HM20B_HUMAN antibody
      • HMG box containing protein 20B antibody
      • HMG box-containing protein 20B antibody
      • HMG domain-containing protein 2 antibody
      • HMG domain-containing protein HMGX2 antibody
      • HMG20B antibody
      • SMARCE1-related protein antibody
      • SMARCE1r antibody
      • sox like antibody
      • Sox like transcriptional factor antibody
      • Sox-like transcriptional factor antibody
      • SOXL antibody
      • Structural DNA binding protein BRAF35 antibody
      • Structural DNA-binding protein BRAF35 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related antibody
      see all

    画像

    • Western blot - Anti-BRAF35 antibody [EPR9915] (ab167415)
      Western blot - Anti-BRAF35 antibody [EPR9915] (ab167415)
      All lanes : Anti-BRAF35 antibody [EPR9915] (ab167415) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody

      Predicted band size: 35 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-BRAF35 antibody [EPR9915] (ab167415)
      Immunocytochemistry/ Immunofluorescence - Anti-BRAF35 antibody [EPR9915] (ab167415)
      Immunofluorescent analysis of A431 cells labeling BRAF35 with ab167415 at 1/100 dilution.
    • Anti-BRAF35 antibody [EPR9915] (ab167415)
      Anti-BRAF35 antibody [EPR9915] (ab167415)

    参考文献 (1)

    ab167415 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab167415 は 1 報の論文で使用されています。

    • McClellan D  et al. Growth Factor Independence 1B-Mediated Transcriptional Repression and Lineage Allocation Require Lysine-Specific Demethylase 1-Dependent Recruitment of the BHC Complex. Mol Cell Biol 39:N/A (2019). PubMed: 30988160

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