Anti-BPGM 抗体 [EPR15128] (ab186739)
![Western blot - Anti-BPGM antibody [EPR15128] (ab186739)](https://www.abcam.co.jp/ps/products/186/ab186739/Images/ab186739-220154-anti-bpgm-antibody-epr15128-western-blot.jpg "Western blot - Anti-BPGM antibody [EPR15128] (ab186739)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15128] to BPGM
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-BPGM antibody [EPR15128]
BPGM 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR15128] to BPGM -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human placenta lysate, Human blood lysate; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR15128 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186739の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/110.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
|
|
| IP |
1/50 - 1/70.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/110. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
|
IP
1/50 - 1/70. |
ターゲット情報
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機能
Plays a major role in regulating hemoglobin oxygen affinity by controlling the levels of 2,3-bisphosphoglycerate (2,3-BPG). Also exhibits mutase (EC 5.4.2.1) and phosphatase (EC 3.1.3.13) activities. -
関連疾患
Defects in BPGM are the cause of bisphosphoglycerate mutase deficiency (BPGMD) [MIM:222800]. A disease characterized by hemolytic anemia, splenomegaly, cholelithiasis and cholecystitis. -
配列類似性
Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily. -
翻訳後修飾
Glycation of Lys-159 in diabetic patients inactivates the enzyme. - Information by UniProt
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参照データベース
- Entrez Gene: 669 Human
- Omim: 613896 Human
- SwissProt: P07738 Human
- Unigene: 198365 Human
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別名
- 2 antibody
- 2,3-bisphosphoglycerate mutase antibody
- 2,3-bisphosphoglycerate mutase, erythrocyte antibody
see all
画像
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Western blot - Anti-BPGM antibody [EPR15128] (ab186739)Anti-BPGM antibody [EPR15128] (ab186739) at 1/10000 dilution + Human blood lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 30 kDa -
![Immunoprecipitation - Anti-BPGM antibody [EPR15128] (ab186739)](https://www.abcam.co.jp/ps/products/186/ab186739/Images/ab186739-220155-anti-bpgm-antibody-epr15128-immunoprecipitation.jpg)
Immunoprecipitation - Anti-BPGM antibody [EPR15128] (ab186739)Western blot analysis on immunoprecipitation from 1) Human blood lysate and 2) PBS, labeling BPGM using 186739 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
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![Western blot - Anti-BPGM antibody [EPR15128] (ab186739)](https://www.abcam.co.jp/ps/products/186/ab186739/Images/ab186739-220153-anti-bpgm-antibody-epr15128-western-blot.jpg)
Western blot - Anti-BPGM antibody [EPR15128] (ab186739)Anti-BPGM antibody [EPR15128] (ab186739) at 1/1000 dilution + Human placenta lysate at 20 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 30 kDa -
![Flow Cytometry (Intracellular) - Anti-BPGM antibody [EPR15128] (ab186739)](https://www.abcam.co.jp/ps/products/186/ab186739/Images/ab186739-220152-anti-bpgm-antibody-epr15128-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-BPGM antibody [EPR15128] (ab186739)Intracellular Flow Cytometry analysis of paraformaldehyde-fixed HeLa cells labeling BPGM with ab186739 at a 1/110 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).
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![Anti-BPGM antibody [EPR15128] (ab186739)](https://www.abcam.co.jp/ps/products/186/ab186739/Images/ab186739-5-benefits-of-recombinant-antibodies.png)
Anti-BPGM antibody [EPR15128] (ab186739)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab186739 は論文での使用が確認できていません。