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Anti-Bovine Serum Albumin 抗体 [5F9] (ab9092)

Submit a review Q&A (6)References (4)
Western blot - Anti-Bovine Serum Albumin antibody [5F9] (ab9092)

    Key features and details

    • Mouse monoclonal [5F9] to Bovine Serum Albumin
    • Suitable for: WB, ELISA
    • Reacts with: Cow
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-Bovine Serum Albumin antibody [5F9]
      Bovine Serum Albumin 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [5F9] to Bovine Serum Albumin

    • 由来種

      Mouse

    • 特異性

      The antibody recognizes a 66 kDa band corresponding to bovine serum albumin monomer. No cross reactivity is detected against other serum proteins by immunoblot. No reactivity is detected by ELISA against human serum albumin or ovalbumin.

    • アプリケーション

      適用あり: WB, ELISAmore details

    • 種交差性

      交差種: Cow
      非交差種: Chicken, Human

    • 免疫原

      Full length native protein (purified) corresponding to Bovine Bovine Serum Albumin.
      Database link: P02769

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      pH: 7.2
      Preservative: 0.01% Sodium azide
      Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
    • Concentration information loading...

    • 精製度

      Protein A purified

    • 特記事項（精製）

      Bovine Serum Albumin monoclonal antibody was purified from concentrated tissue culture supernate by Protein A chromatography and reacts with bovine serum albumin.

    • ポリ/モノ

      モノクローナル

    • クローン名

      5F9

    • ミエローマ

      Sp2/0

    • アイソタイプ

      IgG1

    • 軽鎖の種類

      unknown

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab9092の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/2000 - 1/10000.
    ELISA
    1/120000.
    特記事項
    WB
    1/2000 - 1/10000.
    ELISA
    1/120000.

    ターゲット情報

    • Information by UniProt

    • 参照データベース

      • 別名

        • alb antibody
        • ALBU_HUMAN antibody
        • Albumin (32 AA) antibody
        • Albumin (AA 34) antibody
        • Albumin antibody
        • BSA antibody
        • Cell growth inhibiting protein 42 antibody
        • Growth inhibiting protein 20 antibody
        • PRO0883 antibody
        • PRO0903 antibody
        • PRO1341 antibody
        • Serum albumin antibody
        see all

      画像

      • Western blot - Anti-Bovine Serum Albumin antibody [5F9] (ab9092)
        Western blot - Anti-Bovine Serum Albumin antibody [5F9] (ab9092)

        Anti-Bovine Serum Albumin is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2). In both lanes 20 ul of sample was applied. Primary antibody is diluted 1:5000 in PBS and is reacted for 1.5 h at room temperature.

        Detection occurs using a 1:5000 dilution of IRDye800TM Conjugated Goat-a-Mouse IgG reacted for 1 h at room temperature. Visualization is by near-infrared (IR) western blotting analysis using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR Biosciences, Lincoln, NE.

        Anti-Bovine Serum Albumin is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2). In both lanes 20 ul of sample was applied. Primary antibody is diluted 1:5000 in PBS and is reacted for 1.5 h at room temperature.

        Detection occurs using a 1:5000 dilution of IRDye800

      参考文献 (4)

      ab9092 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab9092 は 4 報の論文で使用されています。

      • Duong K  et al. Immunoaffinity extraction followed by enzymatic digestion for the isolation and identification of proteins employing automated μSPE reactors and mass spectrometry. Anal Bioanal Chem N/A:N/A (2022). PubMed: 36369591
      • Kutikhin AG  et al. Apoptosis-mediated endothelial toxicity but not direct calcification or functional changes in anti-calcification proteins defines pathogenic effects of calcium phosphate bions. Sci Rep 6:27255 (2016). WB . PubMed: 27251104
      • Maloney JA  et al. Molecular mechanisms of Alzheimer disease protection by the A673T allele of amyloid precursor protein. J Biol Chem 289:30990-1000 (2014). PubMed: 25253696
      • Li X  et al. Single-step biocompatible coating for sulfhydryl coupling of receptors using 2-(pyridinyldithio)ethylcarbamoyl dextran. Colloids Surf B Biointerfaces 61:113-7 (2008). PubMed: 17703925

      レビューと Q&A

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      レビューを送る 質問を送る

      1-6 of 6 Abreviews or Q&A

      Question

      Anti BSA antibodies, polyclonal, unconjugated

      Read More
      Answer

      Thank you for contacting us.

      Please type Bovine Serum Album in the search box or click on the following link

      https://www.abcam.com/index.html?pageconfig=searchresults

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

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      Question

      Customer would like to know if this antibody was purified from cell culture or ascites.

      Read More
      Answer

      Ab9092 was purified from ascites.

      Read More

      Question

      Was the BSA antibody (mouse monoclonal [5F9] to Bovine Serum Albumin) derived from ascites or tissue culture?

      Read More
      Answer

      Thank you for your enquiry. We would like to confirm that this antibody derived from ascites.

      Read More

      Question

      I am sorry to say that now I have got a new problem with your antibody ab9092. I have tried to show that I don´t have any bovine serum left in a cell culture, just human serum, with no success.Last week I tested diluted human serum at the same time as diluted bovine serum in a Western blot, both got positive. In your product data sheet it says that the antibody should not cross react with human albumin. Now when it does I can´t use it for the purpose it was meant. I have already bought it twice, because I trusted your information and didn´t check the cross reactivity until now. What shall we do about it?

      Read More
      Answer

      Albumin is the most abundant protein in serum. I don't know what dilutions you did to human serum? The antibody would non-specifically bind to HSA when HSA overwhelmingly presented in the loading sample. I suggest you try to dilute both sera at least 1:1000 and titer down the antibody too.

      Read More

      Question

      I have used your antibody ab9092 in western blot with no success. The gel and blotting are done with methods I normally use. Purified BSA have been included as positive control. The secondary antibody was a rabbit anti- mouse-Ig HRP-conjugated which has worked in other blotting assays. Do you have any idea what is wrong? As blocking buffer I use PBS with 1% Tween 20 and as dilution buffer PBS with 0.05% Tween 20. This is my ordinary protocol for Western blot. Best regards,

      Read More
      Answer

      Thank you for your message. We would suggest trying PBS with 0.1% Tween 20 and 1.5% fish gel as blocking and dilution buffer. Load 20uL of purified BSA at 0.5mg/mL and use 1:1000 dilution of the primary antibody.

      Read More

      Question

      Could you please let me know the protocol or related information for this Ab for example, buffer used, type of BSA coated on the plate, if you use specific type of plate and type of ELISA that you use, that will certainly help us.

      Read More
      Answer

      For the protocol please reference our ELISA under Methods on the web. The BSA we've been using is Fraction V (IgG and Protease free).

      Read More

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