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リコンビナントRabMAb

Anti-BORIS 抗体 [EP12204] (ab187163)

Reviews (1) Submit a question References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] (ab187163)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] (ab187163)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] (ab187163)
  • Anti-BORIS antibody [EP12204] (ab187163)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP12204] to BORIS
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-BORIS antibody [EP12204]
    BORIS 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP12204] to BORIS

  • 由来種

    Rabbit

  • 特異性

    We don't recommend WB application for this antibody as it detects extra bands.

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human, mouse and rat testis tissue.

  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187163の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.

  • 組織特異性

    Testis specific. Specifically expressed in primary spermatocytes.

  • 配列類似性

    Belongs to the CTCF zinc-finger protein family.
    Contains 11 C2H2-type zinc fingers.

  • 発生段階

    Is up-regulated in primary spermatocytes (expression overlapping with the genome-wide erasure of methylation) to become silenced on activation of CTCF in post-meiotic germline cells (expression corresponding to DNA remethylation).

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • BORIS like protein antibody
      • Brother of the regulator of imprinted sites antibody
      • Cancer/testis antigen 27 antibody
      • CCCTC binding factor (zinc finger protein) like antibody
      • CCCTC-binding factor antibody
      • CT27 antibody
      • CTCF paralog antibody
      • CTCF T antibody
      • CTCF-like protein antibody
      • Ctcfl antibody
      • CTCFL_HUMAN antibody
      • dJ579F20.2 antibody
      • HMG 1L1 antibody
      • HMGB1L1 antibody
      • MGC163358 antibody
      • MGC169105 antibody
      • MGC169106 antibody
      • Putative high mobility group protein 1 like 1 antibody
      • Putative high mobility group protein B1 like 1 antibody
      • Transcriptional repressor CTCFL antibody
      • Zinc finger protein CTCF-T antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] (ab187163)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] (ab187163)

      Immunohistochemical analysis of paraffin-embedded human testis tissue labeling BORIS with ab187163 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] (ab187163)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] (ab187163)

      Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling BORIS with ab187163 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] (ab187163)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BORIS antibody [EP12204] (ab187163)

      Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling BORIS with ab187163 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-BORIS antibody [EP12204] (ab187163)
      Anti-BORIS antibody [EP12204] (ab187163)

    参考文献 (3)

    ab187163 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab187163 は 3 報の論文で使用されています。

    • Gong M  et al. Genome-wide bioinformatics analysis reveals CTCFL is upregulated in high-grade epithelial ovarian cancer. Oncol Lett 18:4030-4039 (2019). PubMed: 31516605
    • Debruyne DN  et al. BORIS promotes chromatin regulatory interactions in treatment-resistant cancer cells. Nature 572:676-680 (2019). PubMed: 31391581
    • López-Romero R  et al. The expression of transcription factor BORIS and its association with the estrogen receptor beta (ER-ß) in cervical carcinogenesis. Int J Clin Exp Pathol 12:3208-3221 (2019). PubMed: 31934165

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-BORIS antibody [EP12204]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (KELLY)
    Gel Running Conditions
    Reduced Denaturing (NuPAGE 4-12% Bis-Tris)
    Loading amount
    40 µg
    Specification
    KELLY
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Michael McLane

    Verified customer

    投稿 Apr 13 2018

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