Anti-Borealin/CDCA8 抗体 (ab67126)
Key features and details
- Mouse polyclonal to Borealin/CDCA8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Borealin/CDCA8 antibody
Borealin/CDCA8 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to Borealin/CDCA8 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Full length native Borealin/CDCA8 (purified) (Human)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67126の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
ターゲット情報
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機能
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment. -
配列類似性
Belongs to the borealin family. -
発生段階
Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase. -
ドメイン
The C-terminal region (aa 207-280) represents the dimerization motif. -
翻訳後修飾
Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3. -
細胞内局在
Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells. - Information by UniProt
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参照データベース
- Entrez Gene: 55143 Human
- Omim: 609977 Human
- SwissProt: Q53HL2 Human
- Unigene: 524571 Human
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別名
- BOR antibody
- BOREA_HUMAN antibody
- Borealin antibody
see all
画像
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Western blot - Anti-Borealin/CDCA8 antibody (ab67126)All lanes : Anti-Borealin/CDCA8 antibody (ab67126) at 1/500 dilution
Lane 1 : Borealin/CDCA8 transfected 293T cell lysate
Lane 2 : Untransfected 293T cell lysate
Secondary
All lanes : Goat anti mouse IgG (H&L)-HRP conjugate secondary
antibody at 1/2500 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab67126 は 2 報の論文で使用されています。
- Peng R et al. Identification of Core Genes Involved in the Metastasis of Clear Cell Renal Cell Carcinoma. Cancer Manag Res 12:13437-13449 (2020). PubMed: 33408516
- Pike T et al. PKC? switches Aurora B specificity to exit the abscission checkpoint. Nat Commun 7:13853 (2016). WB, ICC/IF . PubMed: 28004745
