Anti-Bora 抗体 [RP23040151]

• WB

Supplier Data

Western blot - Anti-Bora antibody [RP23040151] (AB308116)

Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), Jurkat (Lane 3), HCT116 (Lane 4), SH-SY5Y (Lane 5), Mouse Heart (Lane 6). The blots were probed with ab308116 at 1-2 µg/mL and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution. A ~ 52 kDa band corresponding to BORA was observed. Known quantity of protein samples were electrophoresed using a 10% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

All lanes:

Western blot - Anti-Bora antibody [RP23040151] (ab308116) at 1 µg/mL

Lane 1:

HeLa cell lysate at 30 µg

Lane 2:

A431 cell lysate at 30 µg

Lane 3:

Jurkat cell lysate at 30 µg

Lane 4:

HCT 116 cell lysate at 30 µg

Lane 5:

SH-SY5Y cell lysate at 30 µg

Lane 6:

Mouse heart tissue lysate at 30 µg

Secondary

All lanes:

HRP conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/2500 dilution

Observed band size: 53 kDa

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