JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB99891

Anti-BOB1 抗体 [SP92]

Anti-BOB1 antibody [SP92]

Be the first to review this product! Submit a review

|

(1 Publication)

Anti-BOB1 antibody [SP92] (ab99891) is a rabbit monoclonal antibody detecting BOB1 in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Trusted since 2010

別名を表示する

BOB1, OBF1, POU2AF1, POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [SP92] (AB99891)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [SP92] (AB99891)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling BOB1 with Purified ab99891 at 1/100 dilution (0.96 μg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 1 (pH 6.0) for 20mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody.

Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [SP92] (AB99891)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [SP92] (AB99891)

Staining of Human BOB1 in a Formalin/PFA-fixed paraffin-embedded section of Human Tonsil using ab99891 at a dilution of 1/100.

Western blot - Anti-BOB1 antibody [SP92] (AB99891)
  • WB

Unknown

Western blot - Anti-BOB1 antibody [SP92] (AB99891)

All lanes:

Western blot - Anti-BOB1 antibody [SP92] (ab99891) at 1/100 dilution

All lanes:

lysate prepared from Ramos cells

Predicted band size: 27 kDa

Observed band size: 35 kDa

false

関連する標識済み抗体及び組成の異なる製品 (8)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

SP92

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.</p>" } } }
style
left-column

製品の詳細

What is this antibody validated in?
Anti-BOB1 antibody [SP92] (ab99891) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

Collaboration
Anti-BOB1 [SP92] (ab99891) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Other related products
We have a range of other formats of antibody clone [SP92] also available for your convenience: ab99891, Carrier free - ab239921, ab279436, Alexa Fluor® 488 - ab320611, Alexa Fluor® 647 - ab320612, Alexa Fluor® 555 - ab320613, Alexa Fluor® 594 - ab320614, PE - ab320615, APC - ab320616, Alexa Fluor® 750 - ab321780

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A/G
精製に関する特記事項
Purified from TCS by protein A/G.
バッファー組成
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
style
left-column
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed : 7859290). It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed : 7779176). It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed : 7779176). It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed : 7623806, PubMed : 7859290). Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).
See full target information POU2AF1
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Modern pathology : an official journal of the Unit 30:1144-1151 PubMed28548127

2017

Mismatch repair deficiency commonly precedes adenoma formation in Lynch Syndrome-Associated colorectal tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shigeki Sekine,Taisuke Mori,Reiko Ogawa,Masahiro Tanaka,Hiroshi Yoshida,Hirokazu Taniguchi,Takeshi Nakajima,Kokichi Sugano,Teruhiko Yoshida,Mamoru Kato,Eisaku Furukawa,Atsushi Ochiai,Nobuyoshi Hiraoka
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB3426

Anti-BOB1 antibody

Western blot - Anti-BOB1 antibody (AB3426)

  • 0
  • 0
Primary Antibodies

AB52103

Anti-BOB1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody (AB52103)

  • 0
  • 0
Primary Antibodies

AB92315

Anti-BOB1 antibody [EPR3299]

RabMAb|Recombinant

Western blot - Anti-BOB1 antibody [EPR3299] (AB92315)

  • 0
  • 0
Primary Antibodies

AB203110

Anti-BOB1 antibody [EPR17685]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] (AB203110)

  • 0
  • 0
Primary Antibodies

AB76010

Anti-BOB1 antibody [EP3300]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-BOB1 antibody [EP3300] (AB76010)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB134929

Anti-Human Kappa Chain antibody [EPR5539-71-8]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (AB134929)

  • 0
  • 0
Primary Antibodies

AB105227

Anti-ICOS antibody [SP98]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (AB105227)

  • 3
  • 2
Primary Antibodies

AB16702

Anti-CD23 antibody [SP23]

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry - Anti-CD23 antibody [SP23] (AB16702)

  • 0
  • 0
Primary Antibodies

AB246518

Anti-CXCL13 antibody [EPR23400-92]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR23400-92] (AB246518)

  • 5
  • 1

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com