Anti-BOB1 抗体 [EP3300] (ab76010)
![Flow Cytometry (Intracellular) - Anti-BOB1 antibody [EP3300] (ab76010)](https://www.abcam.co.jp/ps/products/76/ab76010/Images/ab76010-275024-anti-bob1-antibody-ep3300-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-BOB1 antibody [EP3300] (ab76010)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3300] to BOB1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-BOB1 antibody [EP3300]
BOB1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP3300] to BOB1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
適用なし: ICC/IF or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human BOB1 aa 200-300. The exact sequence is proprietary.
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ポジティブ･コントロール
- Ramos cell lysate. Human tonsil tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
解離定数（KD 値）
KD = 1.14 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP3300 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76010の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
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| WB |
1/5000 - 1/30000. Predicted molecular weight: 28 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
WB
1/5000 - 1/30000. Predicted molecular weight: 28 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. -
組織特異性
B-cell specific. -
関連疾患
Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. -
配列類似性
Belongs to the POU2AF1 family. -
翻訳後修飾
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5450 Human
- Omim: 601206 Human
- SwissProt: Q16633 Human
- Unigene: 654525 Human
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別名
- B cell Oct binding protein 1 antibody
- B cell specific coactivator OBF 1 antibody
- B cell specific coactivator OBF1 antibody
see all
画像
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Flow Cytometry (Intracellular) - Anti-BOB1 antibody [EP3300] (ab76010)
Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling BOB1 with unpurified ab76010 at 1/40 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Western blot - Anti-BOB1 antibody [EP3300] (ab76010)](https://www.abcam.co.jp/ps/products/76/ab76010/Images/ab76010-91290-anti-bob1-antibody-ep3300-western-blot.jpg)
Western blot - Anti-BOB1 antibody [EP3300] (ab76010)Anti-BOB1 antibody [EP3300] (ab76010) at 1/30000 dilution + Ramos cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EP3300] (ab76010)](https://www.abcam.co.jp/ps/products/76/ab76010/Images/ab76010-91289-anti-bob1-antibody-ep3300-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EP3300] (ab76010)ab76010, at 1/100 dilution, staining BOB1 in human tonsil by immunohistochemistry using paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-BOB1 antibody [EP3300] (ab76010)](https://www.abcam.co.jp/ps/products/76/ab76010/Images/ab76010-91288-anti-bob1-antibody-ep3300.jpg)
OI-RD Scanning - Anti-BOB1 antibody [EP3300] (ab76010) -
![Anti-BOB1 antibody [EP3300] (ab76010)](https://www.abcam.co.jp/ps/products/76/ab76010/Images/ab76010-5-benefits-of-recombinant-antibodies.png)
Anti-BOB1 antibody [EP3300] (ab76010)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab76010 は論文での使用が確認できていません。