Anti-BNIP3L/NIX (phospho S81) 抗体 [EPR19826]

• WB

Supplier Data

Western blot - Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] (AB208190)

The molecular weight observed is consistent with what has been described in the literature (PMID : 10467396).

The plasmids were kindly provided by our collaborator Dr. Zhong Chen, Zhejiang University.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Western blot - Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] (ab208190) at 1/5000 dilution

Lane 1:

HEK-293 (human embryonic kidney) transfected with an empty vector (vector control) containing a DDDDK tag, whole cell lysate at 10 µg

Lane 2:

HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX (WT) expression vector, whole cell lysate at 10 µg

Lane 3:

HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX (WT) expression vector, lysate then treated with alkaline phosphatase for 1 hour, whole cell lysate at 10 µg

Lane 4:

HEK-293 transfected with a DDDDK-tagged mouse BNIP3L/NIX S81A mutant expression vector, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 24 kDa

Observed band size: 40 kDa,80 kDa

true

• Dot

Supplier Data

Dot Blot - Anti-BNIP3L/NIX (phospho S81) antibody [EPR19826] (AB208190)

Dot blot analysis of BNIP3L/NIX (phospho S81) labeled with ab208190 at 1/1000 dilution.

Lane 1 : BNIP3L/NIX (phospho S81) peptide;

Lane 2 : BNIP3L/NIX non-phospho peptide;

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.