AB239976

Anti-BNIP3L/NIX 抗体 [EPR4033] - BSA and Azide free

Anti-BNIP3L/NIX antibody [EPR4033] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(2 Publications)

Rabbit Recombinant Monoclonal BNIP3L/NIX antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

別名を表示する

BNIP3A, BNIP3H, NIX, BNIP3L, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like, Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, NIP3-like protein X, NIP3L

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3L/NIX antibody [EPR4033] - BSA and Azide free (AB239976)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3L/NIX antibody [EPR4033] - BSA and Azide free (AB239976)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 1/100 ab109414

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109414).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Western blot - Anti-BNIP3L/NIX antibody [EPR4033] - BSA and Azide free (AB239976)

This data was developed using ab109414, the same antibody clone in a different buffer formulation.

Western blot : Rabbit Monoclonal[EPR4033] to BNIP3L/NIX ab109414 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 26-36 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-BNIP3L/NIX antibody [EPR4033] (<a href='/products/primary-antibodies/bnip3l-nix-antibody-epr4033-ab109414'>ab109414</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 0.1mM CoCl2 24h at 20 µg

Lane 2:

Wild-type A549 Vehicle Control 0mM CoCl2 24h at 20 µg

Lane 3:

BNIP3L knockout A549 0.1mM CoCl2 24h at 20 µg

Lane 4:

BNIP3L knockout A549 Vehicle Control 0mM CoCl2 24h at 20 µg

Lane 5:

HeLa 0.1mM CoCl2 24h at 20 µg

Lane 6:

HeLa Vehicle Control 0mM CoCl2 24h at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 26-36 kDa,76 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4033

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Antigen retrieval is recommended.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

ab239976 is the carrier-free version of ab109414.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP : in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor.

See full target information BNIP3L

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Alzheimer's research & therapy 17:47 PubMed39972387

2025

Cornuside alleviates cognitive impairments induced by Aβ through attenuating NLRP3-mediated neurotoxicity by promoting mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Fulin Zhou,Wenwen Lian,Xiaotang Yuan,Zexing Wang,Congyuan Xia,Yu Yan,Wenping Wang,Zhuohang Tong,Yungchi Cheng,Jiekun Xu,Jun He,Weiku Zhang

Molecules (Basel, Switzerland) 29: PubMed39519747

2024

Co-Mn Complex Oxide Nanoparticles as Potential Reactive Oxygen Species Scavenging Agents for Pulmonary Fibrosis Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Wuhao Yang,Hui Yuan,Hao Sun,Ting Hu,Yaping Xu,Yan Qiu,Yuhang Li

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BNIP3L/NIX 抗体 [EPR4033] - BSA and Azide free

Anti-BNIP3L/NIX antibody [EPR4033] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3L/NIX antibody [EPR4033] - BSA and Azide free (AB239976)

Western blot - Anti-BNIP3L/NIX antibody [EPR4033] - BSA and Azide free (AB239976)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise