Anti-BMP4 抗体 (ab155033)
Key features and details
- Rabbit polyclonal to BMP4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-BMP4 antibody
BMP4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BMP4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to a region within amino acids 1-287 of Human BMP4 (UniProt ID: P12644).
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ポジティブ･コントロール
- Recombinant Human BMP4 protein (ab92855) can be used as a positive control in WB. A549, NIH3T3 and JC whole cell lysates; HeLa cells; Human MDA-MB468 xenograft tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155033の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. -
組織特異性
Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. -
関連疾患
Defects in BMP4 are the cause of microphthalmia syndromic type 6 (MCOPS6) [MIM:607932]; also known as microphthalmia and pituitary anomalies or microphthalmia with brain and digit developmental anomalies. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS6 is characterized by microphthalmia/anophthalmia associated with facial, genital, skeletal, neurologic and endocrine anomalies.
Defects in BMP4 are the cause of non-syndromic orofacial cleft type 11 (OFC11) [MIM:600625]. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. OFC11 is an unusual anomaly consisting of a paramedian scar of the upper lip with an appearance suggesting that a typical cleft lip was corrected in utero. -
配列類似性
Belongs to the TGF-beta family. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 652 Human
- Entrez Gene: 12159 Mouse
- Omim: 112262 Human
- SwissProt: P12644 Human
- SwissProt: P21275 Mouse
- Unigene: 68879 Human
- Unigene: 6813 Mouse
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別名
- zgc:100779 antibody
- BMP 2B antibody
- BMP 4 antibody
see all
画像
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Western blot - Anti-BMP4 antibody (ab155033)Anti-BMP4 antibody (ab155033) at 1/1000 dilution + A549 whole cell lysates at 30 µg
Predicted band size: 47 kDa
10% SDS-PAGE -
Western blot - Anti-BMP4 antibody (ab155033)All lanes : Anti-BMP4 antibody (ab155033) at 1/1000 dilution
Lane 1 : NIH3T3 whole cell lysates
Lane 2 : JC whole cell lysates
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
10% SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-BMP4 antibody (ab155033)Immunofluorescence analysis of methanol fixed HeLa cells, labeling BMP4 using ab155033 at 1/100 dilution. Lower image costained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BMP4 antibody (ab155033)Immunohistochemical analysis of paraffin embedded Human MDA-MB468 xenograft tissue, labeling BMP4 using ab155033 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab155033 は 4 報の論文で使用されています。
- Walker H et al. Conditional Deletion of AP-2β in the Periocular Mesenchyme of Mice Alters Corneal Epithelial Cell Fate and Stratification. Int J Mol Sci 22:N/A (2021). PubMed: 34445433
- Cao W et al. Homeobox a5 Promotes White Adipose Tissue Browning Through Inhibition of the Tenascin C/Toll-Like Receptor 4/Nuclear Factor Kappa B Inflammatory Signaling in Mice. Front Immunol 9:647 (2018). PubMed: 29651293
- Wang G et al. Osteopontin, Bone Morphogenetic Protein-4, and Vitamin D Receptor Gene Polymorphisms in the Susceptibility and Clinical Severity of Spinal Tuberculosis. Cell Physiol Biochem 41:1881-1893 (2017). PubMed: 28376475
- Gao Y et al. WT1 regulates the development of the posterior taste field. Development 141:2271-8 (2014). ICC ; Mouse . PubMed: 24803588