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リコンビナントRabMAb

Anti-BMP15 抗体 [EPR5137] (ab108413)

Reviews (1)Q&A (1)References (5)
Western blot - Anti-BMP15 antibody [EPR5137] (ab108413)
  • Flow Cytometry (Intracellular) - Anti-BMP15 antibody [EPR5137] (ab108413)
  • OI-RD Scanning - Anti-BMP15 antibody [EPR5137] (ab108413)
  • Anti-BMP15 antibody [EPR5137] (ab108413)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5137] to BMP15
  • Suitable for: WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-BMP15 antibody [EPR5137]
    BMP15 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR5137] to BMP15

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Flow Cyt (Intra)more details
    適用なし: ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Ovary cancer, uterus, HL-60, NIH:OVCAR-3, Human testis, and HeLa cell lysates; Hela cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108413の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    May be involved in follicular development. Oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth.

  • 関連疾患

    Defects in BMP15 are the cause of ovarian dysgenesis type 2 (ODG2) [MIM:300510]; also known as X-linked hypergonadotropic ovarian dysgenesis or hypergonadotropic ovarian failure due to ovarian dysgenesis. Ovarian dysgenesis leads to ovarian failure and accounts for about half of the cases of primary amenorrhea.
    Defects in BMP15 are the cause of premature ovarian failure type 4 (POF4) [MIM:300510]. An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol.

  • 配列類似性

    Belongs to the TGF-beta family.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • BMP 15 antibody
      • BMP-15 antibody
      • BMP15 antibody
      • BMP15_HUMAN antibody
      • Bone morphogenetic protein 15 antibody
      • GDF 9B antibody
      • GDF-9B antibody
      • GDF9B antibody
      • Growth/differentiation factor 9B antibody
      • ODG2 antibody
      • POF4 antibody
      see all

    画像

    • Western blot - Anti-BMP15 antibody [EPR5137] (ab108413)
      Western blot - Anti-BMP15 antibody [EPR5137] (ab108413)
      All lanes : Anti-BMP15 antibody [EPR5137] (ab108413) at 1/1000 dilution

      Lane 1 : Ovary cancer tissue lysate
      Lane 2 : Uterus tissue lysate
      Lane 3 : HL-60 cell lysate
      Lane 4 : NIH:OVCAR-3 cell lysate
      Lane 5 : Human testis tissue lysate
      Lane 6 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 45 kDa

    • Flow Cytometry (Intracellular) - Anti-BMP15 antibody [EPR5137] (ab108413)
      Flow Cytometry (Intracellular) - Anti-BMP15 antibody [EPR5137] (ab108413)

      Intracellular flow cytometric analysis of BMP15 in permeabilized Hela cells using ab108413 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

       

    • OI-RD Scanning - Anti-BMP15 antibody [EPR5137] (ab108413)
      OI-RD Scanning - Anti-BMP15 antibody [EPR5137] (ab108413)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-BMP15 antibody [EPR5137] (ab108413)
      Anti-BMP15 antibody [EPR5137] (ab108413)

    参考文献 (5)

    ab108413 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab108413 は 5 報の論文で使用されています。

    • Ghasemi D  et al. Differentiation of human endometrial stem cells encapsulated in alginate hydrogel into oocyte-like cells. Bioimpacts 13:229-240 (2023). PubMed: 37431484
    • Yu X  et al. Oocyte Arrested at Metaphase II Stage were Derived from Human Pluripotent Stem Cells in vitro. Stem Cell Rev Rep 19:1067-1081 (2023). PubMed: 36735215
    • Li D  et al. MicroRNA-375 represses tumor angiogenesis and reverses resistance to sorafenib in hepatocarcinoma. Cancer Gene Ther 28:126-140 (2021). PubMed: 32616906
    • Cheng J  et al. Long-time low-temperature transportation of human ovarian tissue before cryopreservation. Reprod Biomed Online 43:172-183 (2021). PubMed: 34183267
    • Pelosi E  et al. Constitutively active Foxo3 in oocytes preserves ovarian reserve in mice. Nat Commun 4:1843 (2013). Mouse . PubMed: 23673628

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BMP15 antibody [EPR5137]

     Good
    Abreviews
    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Gonad)
    Antigen retrieval step
    Enzymatic
    Permeabilization
    No
    Specification
    Gonad
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 14 2015

    Question

    Inquiry: I'm looking for an A/B they would detect sheep BMP15. Can your tell me the sequence of the peptide used to generate Anti-BMP15 antibody [EPR5137] (ab108413), or maybe check if that a/B would work.

    Read More
    Answer

    Thank you for contacting us. The immunogen sequence has 100% homology with the sheep sequence, so I would expect reactivity. However, this cannot be guaranteed since we have not tested it in sheep.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com