Anti-Bmi1 抗体 (ab38295)
Key features and details
- Rabbit polyclonal to Bmi1
- Suitable for: ICC/IF, WB, IHC-Fr, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Bmi1 antibody
Bmi1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Bmi1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Fr, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow -
免疫原
Synthetic peptide corresponding to 15 amino acids near the center of human Bmi1.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38295の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
Use a concentration of 10 µg/ml.
|
|
WB | (2) |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 37 kDa.
|
IHC-Fr | (1) |
Use at an assay dependent concentration.
|
IP | (1) |
Use at an assay dependent concentration.
|
IHC-P |
Use at an assay dependent concentration. PubMed: 20083111
|
特記事項 |
---|
ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 37 kDa. |
IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. PubMed: 20083111 |
ターゲット情報
-
機能
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. -
配列類似性
Contains 1 RING-type zinc finger. -
翻訳後修飾
Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation. -
細胞内局在
Nucleus. Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 493647 Chicken
- Entrez Gene: 510666 Cow
- Entrez Gene: 100532731 Human
- Entrez Gene: 648 Human
- Entrez Gene: 12151 Mouse
- Entrez Gene: 307151 Rat
- Omim: 164831 Human
- SwissProt: Q5SDR3 Chicken
see all -
別名
- B lymphoma Mo MLV insertion region (mouse) antibody
- B lymphoma Mo MLV insertion region 1 homolog antibody
- Bmi 1 antibody
see all
画像
-
Lane 1 : Anti-Bmi1 antibody (ab38295) at 0.5 µg/ml
Lane 2 : Anti-Bmi1 antibody (ab38295) at 1 µg/ml
Lane 3 : Anti-Bmi1 antibody (ab38295) at 2 µg/ml
All lanes : K562 cell lysate
Predicted band size: 37 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Bmi1 antibody (ab38295)This image is courtesy of an anonymous Abreview
ab38295 staining Bmi1 in Rat fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 2.5% Goat serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 2 hour at 25°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
-
Immunofluorescence of BMI-1 in K562 cells using ab38295 at 20 ug/ml.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (41)
ab38295 は 41 報の論文で使用されています。
- Varlı M et al. 1'-O-methyl-averantin isolated from the endolichenic fungus Jackrogersella sp. EL001672 suppresses colorectal cancer stemness via sonic Hedgehog and Notch signaling. Sci Rep 13:2811 (2023). PubMed: 36797277
- Park KW et al. Establishment of intestinal organoids from small intestine of growing cattle (12 months old). J Anim Sci Technol 64:1105-1116 (2022). PubMed: 36812001
- Raina K et al. Stage-Specific Effect of Inositol Hexaphosphate on Cancer Stem Cell Pool during Growth and Progression of Prostate Tumorigenesis in TRAMP Model. Cancers (Basel) 14:N/A (2022). PubMed: 36077751
- Jiang M et al. Hypoxic exposure activates the B cell-specific Moloney murine leukaemia virus integration site 1/PI3K/Akt axis and promotes EMT in leukaemia stem cells. Oncol Lett 21:98 (2021). PubMed: 33376531
- Jia J et al. Transcriptional factor FoxM1-activated microRNA-335-3p maintains the self-renewal of neural stem cells by inhibiting p53 signaling pathway via Fmr1. Stem Cell Res Ther 12:169 (2021). PubMed: 33691791