Anti-Bmf 抗体 (ab9655)
Key features and details
- Rabbit polyclonal to Bmf
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Bmf antibody -
製品の詳細
Rabbit polyclonal to Bmf -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide:
EPSQCVEELEDDV
, corresponding to amino acids 2-14 of Human Bmf. (Peptide available as ab9656). -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9655の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa.
|
| ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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| IHC-P |
Use at an assay dependent concentration. PubMed: 14668207
Note: this was tested against sections embedded in polyester wax. |
| 特記事項 |
|---|
|
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
IHC-P
Use at an assay dependent concentration. PubMed: 14668207 Note: this was tested against sections embedded in polyester wax. |
ターゲット情報
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機能
May play a role in apoptosis. Isoform 1 seems to be the main initiator. -
組織特異性
Isoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells. -
配列類似性
Belongs to the Bcl-2 family. - Information by UniProt
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参照データベース
- Entrez Gene: 90427 Human
- Entrez Gene: 171543 Mouse
- Entrez Gene: 246142 Rat
- Omim: 606266 Human
- SwissProt: Q96LC9 Human
- SwissProt: Q91ZE9 Mouse
- SwissProt: Q8K589 Rat
- Unigene: 591104 Human
see all -
別名
- Bcl 2 modifying factor antibody
- Bcl-2-modifying factor antibody
- Bcl2 modifying factor antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)Immunocytochemistry/Immunofluorescence of HeLa cells labeling Bmf with Anti-Bmf antibody (ab9655) at 5μg/ml.
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Western blot - Anti-Bmf antibody (ab9655)Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2
Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2 µg /ml.µ g /ml. -
Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (ab9655)ab9655 at 10µg/ml staining Bmf in human Hela cells by Immunocytochemistry.
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Western blot - Anti-Bmf antibody (ab9655)
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (11)
ab9655 は 11 報の論文で使用されています。
- Zhong Y et al. LncRNA RP11-521C20.3 Inhibits Cigarette Smoke Extract-Induced Apoptosis in A549 Cells by Targeting BMF Signaling. Int J Chron Obstruct Pulmon Dis 18:669-682 (2023). PubMed: 37114104
- Lin X et al. BMF-AS1/BMF Promotes Diabetic Vascular Calcification and Aging both In Vitro and In Vivo. Aging Dis 14:170-183 (2023). PubMed: 36818559
- Hu QG et al. MiR-183-5p-PNPT1 Axis Enhances Cisplatin-induced Apoptosis in Bladder Cancer Cells. Curr Med Sci 42:785-796 (2022). PubMed: 35788944
- Lin X et al. lncRNA-ES3/miR-34c-5p/BMF axis is involved in regulating high-glucose-induced calcification/senescence of VSMCs. Aging (Albany NY) 11:523-535 (2019). PubMed: 30654331
- Kabir S et al. The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells. Elife 8:N/A (2019). PubMed: 31294695
