AB9655

Anti-Bmf 抗体

Name: Anti-Bmf 抗体 (ab9655) | Abcam
Brand: Abcam Limited
SKU: AB9655
Price: 78000 JPY
Availability: InStock
Rating: 3 (4 reviews)

Anti-Bmf antibody

(4 Reviews)

(13 Publications)

Rabbit Polyclonal BMF antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human Bcl-2-modifying factor aa 1-50.

別名を表示する

Bcl-2-modifying factor, BMF

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (AB9655)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (AB9655)

Immunocytochemistry/Immunofluorescence of HeLa cells labeling Bmf with Anti-Bmf antibody (ab9655) at 5μg/ml.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (AB9655)

ab9655 at 10μg/ml staining Bmf in human Hela cells by Immunocytochemistry.

Unknown

Western blot - Anti-Bmf antibody (AB9655)

Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2 μg /ml. Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates with anti-Bmf at 2 µg /ml.

All lanes:

Western blot - Anti-Bmf antibody (ab9655)

Predicted band size: 21 kDa

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Western blot - Anti-Bmf antibody (AB9655)

All lanes:

Western blot - Anti-Bmf antibody (ab9655) at 2 µg/mL

All lanes:

Western blot - Recombinant Human Bmf protein (<a href='/products/proteins-peptides/recombinant-human-bmf-protein-ab85485'>ab85485</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 21 kDa

true

Exposure time: 8min

Unknown

Western blot - Anti-Bmf antibody (AB9655)

All lanes:

Western blot - Anti-Bmf antibody (ab9655) at 2 µg/mL

All lanes:

Western blot - Recombinant Human Bmf protein (<a href='/products/proteins-peptides/recombinant-human-bmf-protein-ab85485'>ab85485</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 21 kDa

true

Exposure time: 8min

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human, Rat, Mouse

アプリケーション

WB, IHC-P, ICC/IF

applications

免疫原

Synthetic Peptide within Human Bcl-2-modifying factor aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q96LC9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Note: this was tested against sections embedded in polyester wax.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Note: this was tested against sections embedded in polyester wax.", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Note: this was tested against sections embedded in polyester wax.", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.2 Preservative: 0.02% Sodium azide

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Bmf also known as Bcl-2 modifying factor is important in apoptosis regulation a process of programmed cell death. It acts as a pro-apoptotic protein from the Bcl-2 family. The Bmf protein has a molecular weight of approximately 21 kDa. It is mainly expressed in various epithelial tissues and abundant in hematopoietic cells. Bmf can interact with the dynein light chain at the cytoskeleton which affects its localization and function.

Biological function summary

The protein exerts its function by promoting apoptosis. Bmf binds directly to pro-survival Bcl-2 family members such as Bcl-X_L and Bcl-2 displacing pro-apoptotic proteins like Bax or Bak. This action facilitates the release of cytochrome c from mitochondria triggering caspase activation and cell death. Bmf can function independently but also works within larger complexes affecting processes related to cellular homeostasis.

Pathways

Bmf operates prominently within the intrinsic apoptotic pathway where it modulates mitochondrial outer membrane permeability. Its action is closely associated with Bax and Bak proteins facilitating their oligomerization. Additionally Bmf integrates into stress-induced signaling pathways responding to signals like oxidative stress or deprivation of survival factors. Through these connections Bmf influences cell fate and stress responses often cross-talking with the JNK signaling pathway.

Bmf has implications in several types of cancer and neurodegenerative conditions. Overexpression or dysregulation of Bmf can contribute to tumor development due to impaired apoptosis. Specific cancers like lymphomas show altered Bmf levels linking it to pathways with proteins such as Bcl-2 and Bax. In neurodegenerative diseases changes in Bmf expression can impact neuronal survival with implications for conditions like Alzheimer's where its interaction with other Bcl-2 family proteins affects cell viability.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

May play a role in apoptosis. Isoform 1 seems to be the main initiator.

See full target information Bcl-2-modifying factor

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:566 PubMed40186004

2025

Glucocorticoids regulate the expression of Srsf1 through Hdac4/Foxc1 axis to induce apoptosis of osteoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Luo,Tao Wang,Zhihong Xie,Fanchao Li,Chengyou Yang,Wentao Dong,Jianhua Wu,Qiang Wang,Fengyang Xu,Jiong Liu,Fei Zhang,Wuxun Peng

Cell death and differentiation 32:1153-1166 PubMed39994353

2025

Collaborative orchestration of BH3-only proteins governs Bak/Bax-dependent hepatocyte apoptosis under antiapoptotic protein-deficiency in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shinnosuke Kudo,Hayato Hikita,Yoshinobu Saito,Kazuhiro Murai,Takahiro Kodama,Tomohide Tatsumi,Tetsuo Takehara

International journal of chronic obstructive pulmonary disease 18:669-682 PubMed37114104

2023

LncRNA RP11-521C20.3 Inhibits Cigarette Smoke Extract-Induced Apoptosis in A549 Cells by Targeting BMF Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Zhong,Chuntao Li,Yaling Xiang,Jinbiao Zhou,Jianqing Zhang

Aging and disease 14:170-183 PubMed36818559

2023

BMF-AS1/BMF Promotes Diabetic Vascular Calcification and Aging both and .

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Lin,Qun-Yan Xiang,Shuang Li,Wan-Ling Song,Yan-Jiao Wang,Yu-Qing Ni,Yan Zhao,Chen Li,Yi Wang,Hua-Hua Li,Zhen Liang,Jun-Kun Zhan,You-Shuo Liu

Current medical science 42:785-796 PubMed35788944

2022

MiR-183-5p-PNPT1 Axis Enhances Cisplatin-induced Apoptosis in Bladder Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Gang Hu,Zhi Yang,Jia-Wei Chen,Gallina Kazobinka,Liang Tian,Wen-Cheng Li

eLife 8: PubMed31294695

2019

The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shaheen Kabir,Justin Cidado,Courtney Andersen,Cortni Dick,Pei-Chun Lin,Therese Mitros,Hong Ma,Seung Hyun Baik,Matthew A Belmonte,Lisa Drew,Jacob E Corn

Aging 11:523-535 PubMed30654331

2019

lncRNA-ES3/miR-34c-5p/BMF axis is involved in regulating high-glucose-induced calcification/senescence of VSMCs.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Lin,Jun-Kun Zhan,Jia-Yu Zhong,Yan-Jiao Wang,Yi Wang,Shuang Li,Jie-Yu He,Pan Tan,Yi-Yin Chen,Xue-Bin Liu,Xing-Jun Cui,You-Shuo Liu

The Journal of experimental medicine 213:3007-3024 PubMed27899442

2016

Early generated B1 B cells with restricted BCRs become chronic lymphocytic leukemia with continued c-Myc and low Bmf expression.

Applications

Unspecified application

Species

Unspecified reactive species

Kyoko Hayakawa,Anthony M Formica,Joni Brill-Dashoff,Susan A Shinton,Daiju Ichikawa,Yan Zhou,Herbert C Morse,Richard R Hardy

International journal of oncology 47:1819-28 PubMed26412386

2015

The inhibition of histone deacetylase 8 suppresses proliferation and inhibits apoptosis in gastric adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyuan Song,Ying Wang,Po Xu,Ruina Yang,Zhikun Ma,Shuo Liang,Guangping Zhang

Oncology reports 32:1200-10 PubMed24993451

2014

Bioinformatics analysis identifies miR-221 as a core regulator in hepatocellular carcinoma and its silencing suppresses tumor properties.

Applications

Unspecified application

Species

Unspecified reactive species

Xing-Xing He,An-Yuan Guo,Chuan-Rui Xu,Ying Chang,Guang-Ya Xiang,Jing Gong,Zi-Li Dan,De-An Tian,Jia-Zhi Liao,Ju-Sheng Lin

View all publications

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-Bmf 抗体

Anti-Bmf antibody

Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (AB9655)

Immunocytochemistry/ Immunofluorescence - Anti-Bmf antibody (AB9655)

Western blot - Anti-Bmf antibody (AB9655)

Western blot - Anti-Bmf antibody (AB9655)

Western blot - Anti-Bmf antibody (AB9655)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (13)

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise