Anti-Blooms Syndrome Protein Blm (phospho T99) 抗体 (ab62206)
Key features and details
- Rabbit polyclonal to Blooms Syndrome Protein Blm (phospho T99)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Blooms Syndrome Protein Blm (phospho T99) antibody
Blooms Syndrome Protein Blm 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Blooms Syndrome Protein Blm (phospho T99) -
由来種
Rabbit -
特異性
ab62206 detects endogenous levels of Bloom Syndrome Protein Blm only when phosphorylated at threonine 99. -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Blooms Syndrome Protein Blm aa 50-150 (phospho T99).
Database link: P54132 -
ポジティブ･コントロール
- HeLa,HepG2 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab62206 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62206の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 159 kDa.
|
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| IHC-P |
1/50 - 1/100.
|
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| ICC/IF |
1/100 - 1/500.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 159 kDa. |
|
IHC-P
1/50 - 1/100. |
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ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. -
関連疾患
Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability. -
配列類似性
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain. -
翻訳後修飾
Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 641 Human
- Omim: 604610 Human
- SwissProt: P54132 Human
- Unigene: 725208 Human
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別名
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
see all
画像
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Western blot - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)All lanes : Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate with blocking peptide
Predicted band size: 159 kDa
Observed band size: 159 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)Immunofluorescence analysis of HeLa cells using Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206) at 1/500 dilution, in the presence (right) or absence (left) of immunising phosphopeptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)ab62206 staining Blooms Syndrome Protein Blm (phospho T99) in Human heart by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The picture on the right is treated with the synthesized peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab62206 は 2 報の論文で使用されています。
- Guo L et al. Random-forest algorithm based biomarkers in predicting prognosis in the patients with hepatocellular carcinoma. Cancer Cell Int 20:251 (2020). PubMed: 32565735
- Tripathi V et al. MRN complex-dependent recruitment of ubiquitylated BLM helicase to DSBs negatively regulates DNA repair pathways. Nat Commun 9:1016 (2018). PubMed: 29523790