AB62206

Anti-Blooms Syndrome Protein Blm (phospho T99) 抗体

Name: Anti-Blooms Syndrome Protein Blm (phospho T99) 抗体 (ab62206) | Abcam
Brand: Abcam Limited
SKU: AB62206
Price: 78000 JPY
Availability: InStock

Anti-Blooms Syndrome Protein Blm (phospho T99) antibody

(1 Review)

(2 Publications)

Rabbit Polyclonal Blooms Syndrome Protein Blm phospho T99 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human BLM pT99 aa 50-150.

別名を表示する

RECQ2, RECQL3, BLM, RecQ-like DNA helicase BLM, Bloom syndrome protein, DNA 3'-5' helicase BLM, RecQ protein-like 3, RecQ2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

ab62206 staining Blooms Syndrome Protein Blm (phospho T99) in Human heart by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The picture on the right is treated with the synthesized peptide.

Immunocytochemistry/ Immunofluorescence - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

Immunofluorescence analysis of HeLa cells using Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206) at 1/500 dilution, in the presence (right) or absence (left) of immunising phosphopeptide.

Unknown

Western blot - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

All lanes:

Western blot - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)

Lane 1:

HepG2 cell lysate

Lane 2:

HepG2 cell lysate with blocking peptide

Predicted band size: 159 kDa

Observed band size: 159 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P, ICC/IF

applications

免疫原

Synthetic Peptide within Human BLM pT99 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P54132

特異性

ab62206 detects endogenous levels of Bloom Syndrome Protein Blm only when phosphorylated at threonine 99.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab62206 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Blooms Syndrome Protein often abbreviated as BLM functions as a DNA helicase with a mass of approximately 141 kDa. BLM unwinds the DNA duplex important for genomic stability during replication and repair processes. This protein sometimes referred to as Bloom's Protein is expressed in various human tissues with higher levels in proliferating cells. BLM participates actively in the maintenance of genome integrity by resolving recombination intermediates and preventing crossover events.

Biological function summary

The BLM helicase acts as a critical player in the maintenance of chromosomal stability. It forms part of a complex known as the BLM complex which includes topoisomerase IIIα RMI1 and RMI2. This complex participates in homologous recombination repair ensuring proper disjunction of intertwined DNA and preventing excessive sister chromatid exchanges. This function is essential for preventing genomic instability which may lead to cell dysfunction or malignancies.

Pathways

The BLM protein associates with the DNA damage response and homologous recombination repair pathways. BLM interacts directly with components of the BRCA1-associated genome surveillance complex and indirectly with the ATM-dependent DNA damage signaling pathways to coordinate DNA repair processes. Its cooperation with proteins like RAD51 is integral for efficient DNA repair and recovery during replication stress conditions.

Bloom Syndrome directly relates to mutations or dysfunction in BLM. This rare genetic disorder leads to increased susceptibility to various cancers due to high rates of genomic instability and sister chromatid exchanges. In addition anomalies in BLM function may connect it to Werner syndrome where increased genomic instability is observed. Both conditions highlight BLM's role in safeguarding genome integrity and its critical interactions with proteins such as WRN helicase affecting cellular aging and carcinogenesis pathways.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

ATP-dependent DNA helicase that unwinds double-stranded (ds)DNA in a 3'-5' direction (PubMed : 24816114, PubMed : 25901030, PubMed : 9388193, PubMed : 9765292). Participates in DNA replication and repair (PubMed : 12019152, PubMed : 21325134, PubMed : 23509288, PubMed : 34606619). Involved in 5'-end resection of DNA during double-strand break (DSB) repair : unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed : 21325134). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution (PubMed : 25901030). Binds single-stranded DNA (ssDNA), forked duplex DNA and Holliday junction DNA (PubMed : 20639533, PubMed : 24257077, PubMed : 25901030). Unwinds G-quadruplex DNA; unwinding occurs in the 3'-5' direction and requires a 3' single-stranded end of at least 7 nucleotides (PubMed : 18426915, PubMed : 9765292). Helicase activity is higher on G-quadruplex substrates than on duplex DNA substrates (PubMed : 9765292). Telomeres, immunoglobulin heavy chain switch regions and rDNA are notably G-rich; formation of G-quadruplex DNA would block DNA replication and transcription (PubMed : 18426915, PubMed : 9765292). Negatively regulates sister chromatid exchange (SCE) (PubMed : 25901030). Recruited by the KHDC3L-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks (By similarity).. (Microbial infection) Eliminates nuclear HIV-1 cDNA, thereby suppressing immune sensing and proviral hyper-integration.

See full target information BLM pT99

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 20:251 PubMed32565735

2020

Random-forest algorithm based biomarkers in predicting prognosis in the patients with hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyun Guo,Zhenjiang Wang,Yuanyuan Du,Jie Mao,Junqiang Zhang,Zeyuan Yu,Jiwu Guo,Jun Zhao,Huinian Zhou,Haitao Wang,Yanmei Gu,Yumin Li

Nature communications 9:1016 PubMed29523790

2018

MRN complex-dependent recruitment of ubiquitylated BLM helicase to DSBs negatively regulates DNA repair pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Vivek Tripathi,Himanshi Agarwal,Swati Priya,Harish Batra,Priyanka Modi,Monica Pandey,Dhurjhoti Saha,Sathees C Raghavan,Sagar Sengupta

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Blooms Syndrome Protein Blm (phospho T99) 抗体

Anti-Blooms Syndrome Protein Blm (phospho T99) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

Immunocytochemistry/ Immunofluorescence - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

Western blot - Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (AB62206)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise