Anti-Blooms Syndrome Protein Blm 抗体 (ab476)
Key features and details
- Rabbit polyclonal to Blooms Syndrome Protein Blm
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Blooms Syndrome Protein Blm antibody
Blooms Syndrome Protein Blm 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Blooms Syndrome Protein Blm -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mammals
-
免疫原
Recombinant fragment (His-tag) corresponding to Human Blooms Syndrome Protein Blm (C terminal). His-tagged 376 amino acid C-terminal of human blm fusion protein.
-
ポジティブ･コントロール
- HeLa nuclear extract.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab476の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa). We have conflicting reports from customers about whether this antibody works in IF in HeLa or SK-N-SH cells. We would appreciate any customer feedback about this antibody.
|
|
| IP |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
1/2000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa). We have conflicting reports from customers about whether this antibody works in IF in HeLa or SK-N-SH cells. We would appreciate any customer feedback about this antibody. |
|
IP
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. -
関連疾患
Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability. -
配列類似性
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain. -
翻訳後修飾
Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 641 Human
- Omim: 604610 Human
- SwissProt: P54132 Human
- Unigene: 725208 Human
-
別名
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
see all
画像
-
Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab476)
Western blot using ab476 against HELA nuclear extract revealing the 160kD Blm protein which, in gels, moves as a 190kD band.
Western blot using ab476 against HELA nuclear extract revealing the 160kD Blm protein which, in gels, moves as a 190kD band. -
Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab476)Image from S Raynard et al, J Biol Chem 281:13861-4 (2006), Fig 2.BLAP75 physically interacts with BLM and Topo IIIa. Purified BLM was incubated with purified Topo IIIa and the reaction mixture was subjected to Immunoprecipitation with ab476. The reaction supernatant (S), wash (W), and eluate (E) were analyzed by SDS-PAGE, GST-BLAP75 or GST alone was incubated with BLM. Protein complexes were captured on glutathione-Sepharose beads, followed by SDS-PAGE.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (35)
ab476 は 35 報の論文で使用されています。
- Rosso I et al. Alternative lengthening of telomeres (ALT) cells viability is dependent on C-rich telomeric RNAs. Nat Commun 14:7086 (2023). PubMed: 37925537
- Balbo Pogliano C et al. The CDK1-TOPBP1-PLK1 axis regulates the Bloom's syndrome helicase BLM to suppress crossover recombination in somatic cells. Sci Adv 8:eabk0221 (2022). PubMed: 35119917
- Ovejero S et al. The BLM helicase is a new therapeutic target in multiple myeloma involved in replication stress survival and drug resistance. Front Immunol 13:983181 (2022). PubMed: 36569948
- Wu C et al. USP37 regulates DNA damage response through stabilizing and deubiquitinating BLM. Nucleic Acids Res 49:11224-11240 (2021). PubMed: 34606619
- Fujiwara C et al. Cell-based chemical fingerprinting identifies telomeres and lamin A as modifiers of DNA damage response in cancer cells. Sci Rep 8:14827 (2018). PubMed: 30287851