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  1. Link

    products/primary-antibodies/blooms-syndrome-protein-blm-antibody-ab2179.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Homologous Recomb.
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Anti-Blooms Syndrome Protein Blm 抗体 (ab2179)

  • Datasheet
  • SDS
Reviews (12)Q&A (5)References (49)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
  • Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
  • Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)

Key features and details

  • Rabbit polyclonal to Blooms Syndrome Protein Blm
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

こちらの製品もご検討ください

標識
Antibody Concentration Kit (Gold/Europium labelling) (ab204911)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
一次抗体
Product image
Anti-Ube2N / Ubc13 antibody (ab25885)

関連製品

製品の概要

  • 製品名

    Anti-Blooms Syndrome Protein Blm antibody
    Blooms Syndrome Protein Blm 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Blooms Syndrome Protein Blm
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IPmore details
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • WB: HEK-293T, K-562, TCMK-1, NIH/3T3 and Jurkat whole cell lysate. IP: Blooms Syndrome Protein Blm in HEK-293T whole cell lysate.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7
    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2179の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (6)
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa).
IP (1)
Use at 2-10 µg/mg of lysate.
特記事項
WB
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa).
IP
Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能

    Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
  • 関連疾患

    Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
  • 配列類似性

    Belongs to the helicase family. RecQ subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.
  • 翻訳後修飾

    Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession P54132 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 641 Human
    • Entrez Gene: 12144 Mouse
    • Omim: 604610 Human
    • SwissProt: P54132 Human
    • SwissProt: O88700 Mouse
    • Unigene: 725208 Human
    • 別名

      • Blm antibody
      • BLM_HUMAN antibody
      • Bloom syndrome antibody
      • Bloom syndrome protein antibody
      • Bloom syndrome RecQ helicase like antibody
      • BS antibody
      • DNA helicase antibody
      • DNA helicase RecQ like type 2 antibody
      • MGC126616 antibody
      • MGC131618 antibody
      • MGC131620 antibody
      • RECQ 2 antibody
      • RECQ like antibody
      • RecQ like type 2 antibody
      • RecQ protein like 3 antibody
      • RecQ protein-like 3 antibody
      • RecQ-like type 2 antibody
      • RecQ2 antibody
      • RECQL 2 antibody
      • RECQL 3 antibody
      • RECQL2 antibody
      • RECQL3 antibody
      • type 2 antibody
      see all

    画像

    • Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
      Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
      All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab2179) at 0.1 µg/ml

      Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 5 : A549 (Human lung carcinoma cell line) whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 159 kDa


      Exposure time: 30 seconds


      Lysates prepared using NETN lysis buffer.

    • Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
      Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab2179)

      Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK-293T cells prepared using NETN lysis buffer.

      Antibodies: ab2179 used for IP at 6 µg per reaction.

      Two lots tested (Lanes 1 and 2).

      Lane 3 is Control IgG.

      For blotting immunoprecipitated BLM, ab2179 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

    • Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
      Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)
      All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab2179) at 0.1 µg/ml

      Lane 1 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
      Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 159 kDa


      Exposure time: 75 seconds


      Lysates prepared using NETN lysis buffer.

    プロトコール

    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (49)

    ab2179 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2179 は 49 報の論文で使用されています。

    • Guo Y  et al. BLM promotes malignancy in PCa by inducing KRAS expression and RhoA suppression via its interaction with HDGF and activation of MAPK/ERK pathway. J Cell Commun Signal 17:757-772 (2023). PubMed: 36574142
    • Kong N  et al. RIF1 suppresses the formation of single-stranded ultrafine anaphase bridges via protein phosphatase 1. Cell Rep 42:112032 (2023). PubMed: 36719798
    • Maresca C  et al. PARP1 allows proper telomere replication through TRF1 poly (ADP-ribosyl)ation and helicase recruitment. Commun Biol 6:234 (2023). PubMed: 36864251
    • Rogers CB  et al. Fanconi anemia-associated chromosomal radial formation is dependent on POLθ-mediated alternative end joining. Cell Rep 42:112428 (2023). PubMed: 37086407
    • Balbo Pogliano C  et al. The CDK1-TOPBP1-PLK1 axis regulates the Bloom's syndrome helicase BLM to suppress crossover recombination in somatic cells. Sci Adv 8:eabk0221 (2022). PubMed: 35119917
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 17 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-Blooms Syndrome Protein Blm antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (hek293)
    Total protein in input
    300 µg
    Immuno-precipitation step
    Other - protein G dynabeads, 10 microliters
    Specification
    hek293
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Edyta Marcon

    Verified customer

    投稿 Apr 30 2015

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Non-reduced Denaturing (8%)
    Sample
    Hamster Cell lysate - whole cell (Chinese Hamster Ovary cells (TRex CHO ))
    Specification
    Chinese Hamster Ovary cells (TRex CHO )
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Eleni Petsalaki

    Verified customer

    投稿 Feb 05 2014

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (NTERA-2)
    Loading amount
    25 µg
    Specification
    NTERA-2
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Feb 05 2013

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (ntera2)
    Loading amount
    60000 cells
    Specification
    ntera2
    Treatment
    siRNA of Bloom
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Faisal Alzahrani

    Verified customer

    投稿 Oct 18 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Blooms Syndrome Protein Blm antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Colon carcinoma cells)
    Specification
    Colon carcinoma cells
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Eleni Petsalaki

    Verified customer

    投稿 Oct 08 2012

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Non-reduced Denaturing (8% gel)
    Sample
    Human Cell lysate - whole cell (Colon carcinoma)
    Specification
    Colon carcinoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Eleni Petsalaki

    Verified customer

    投稿 Oct 08 2012

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse testis)
    Loading amount
    100 µg
    Specification
    mouse testis
    Treatment
    Irradiation - 20 gy
    Gel Running Conditions
    Reduced Denaturing (8% Precast gel (Invitrogen))
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 12 2012

    Immunocytochemistry abreview for Anti-Blooms Syndrome Protein Blm antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (mouse testis)
    Specification
    mouse testis
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 12 2012

    Immunocytochemistry abreview for Anti-Blooms Syndrome Protein Blm antibody

    Average
    Abreviews
    Abreviews
    Application
    Immunocytochemistry
    Sample
    Human Cell (HEK293-T)
    Specification
    HEK293-T
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 05 2011

    Question

    DESCRIPTION OF THE PROBLEM Non-specific staining
    SAMPLE Immortalized Human Bloom Syndrome cell line (6040 hTERT)
    PRIMARY ANTIBODY ab2179 dilutions, 1:100, 1:200, and 1:400 incubated 1hr, wash: 3X 5 min in 0.2% tween-20 in pbs
    DETECTION METHOD Fluorescent microscope
    POSITIVE AND NEGATIVE CONTROLS USED Positive Control: BJ hTERT (WT) Negative Control: 6040 hTERT (BS line)
    ANTIBODY STORAGE CONDITIONS 4C
    FIXATION OF SAMPLE 4% Paraformaldehyde
    PERMEABILIZATION STEP 1min in .2% Tween-20 in PBS
    BLOCKING CONDITIONS Blocking Buffer: 1% BSA, 0.2% tween-20, 0.02% azide in PBS 30 min at 37C
    SECONDARY ANTIBODY Invitrogen: Alexa Fluor 488 goat anti-rabbit IgG 1:400 dilution, 1hr at 37C wash: 3X 5 min in 0.2% tween-20 in pbs
    HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1
    HAVE YOU RUN A NO PRIMARY CONTROL? Yes
    DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes
    ADDITIONAL NOTES I've attached a picture of the IF I obtained on my BS cell line (negative control) Blue: dapi, Green: Blm ab2179. I've looked through the literature and haven't been able to find anyone using any of the BLM antibodies against a BS cell line. Has this or any of the BLM antibodies been tested on Bloom Syndrome cell lines?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 13 2012

    Answer

    Thank you for contacting Abcam regarding ab2179.

    I am sorry that you have been experiencing difficulties with this antibody in ICC. I have reviewed the protocol information you provided and would like to make some suggestions to improve your results with this antibody.

    Regarding your question about your cells, at this time we have not tested nor received feedback from anyone who has used this antibody in a Bloom Syndrome cell line. As a positive control, I would recommend testing HeLa cells treated with 15uM Bleomycin for 2 hours as this produces a positive result with the antibody.

    In terms of protocol modifications, I would recommend the following:

    Since this is a nuclear protein, I would recommend triton X-100 to permeabilize - try 0.1% in PBS for 10 min. If you continue to use Tween-20 I would increase this to at least 10 min. Alternatively I would recommend fixation with acetone, which will both fix and permeabilize the cells.

    The blocking step seems sufficient, but I would increase the incubation with the primary antibody to at least 2 hours, although ideally overnight at 4oC.

    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions or if these suggestions do not improve your results with this antibody. The antibody is guaranteed under our Abpromise for WB and I would be happy to offer a replacement or credit if we determine the antibody is not working properly.

    Read More

    Abcam Scientific Support

    Answered on Mar 13 2012

    1-10 of 17 Abreviews or Q&A

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