Anti-Blooms Syndrome Protein Blm 抗体 (ab2179)
Key features and details
- Rabbit polyclonal to Blooms Syndrome Protein Blm
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Blooms Syndrome Protein Blm antibody
Blooms Syndrome Protein Blm 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Blooms Syndrome Protein Blm -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HEK-293T, K-562, TCMK-1, NIH/3T3 and Jurkat whole cell lysate. IP: Blooms Syndrome Protein Blm in HEK-293T whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2179の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (6) |
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa).
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| IP | (1) |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa). |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. -
関連疾患
Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability. -
配列類似性
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain. -
翻訳後修飾
Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 641 Human
- Entrez Gene: 12144 Mouse
- Omim: 604610 Human
- SwissProt: P54132 Human
- SwissProt: O88700 Mouse
- Unigene: 725208 Human
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別名
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
see all
画像
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Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab2179) at 0.1 µg/ml
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 159 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab2179)Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK-293T cells prepared using NETN lysis buffer.
Antibodies: ab2179 used for IP at 6 µg per reaction.
Two lots tested (Lanes 1 and 2).
Lane 3 is Control IgG.
For blotting immunoprecipitated BLM, ab2179 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab2179)All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab2179) at 0.1 µg/ml
Lane 1 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 159 kDa
Exposure time: 75 secondsLysates prepared using NETN lysis buffer.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (45)
ab2179 は 45 報の論文で使用されています。
- Balbo Pogliano C et al. The CDK1-TOPBP1-PLK1 axis regulates the Bloom's syndrome helicase BLM to suppress crossover recombination in somatic cells. Sci Adv 8:eabk0221 (2022). PubMed: 35119917
- Cohen S et al. A POLD3/BLM dependent pathway handles DSBs in transcribed chromatin upon excessive RNA:DNA hybrid accumulation. Nat Commun 13:2012 (2022). PubMed: 35440629
- Jiang W et al. Predominant cellular mitochondrial dysfunction in the TOP3A gene-caused Bloom syndrome-like disorder. Biochim Biophys Acta Mol Basis Dis 1867:166106 (2021). PubMed: 33631320
- Hambarde S et al. EXO5-DNA structure and BLM interactions direct DNA resection critical for ATR-dependent replication restart. Mol Cell 81:2989-3006.e9 (2021). PubMed: 34197737
- Yan S et al. ZGRF1 promotes end resection of DNA homologous recombination via forming complex with BRCA1/EXO1. Cell Death Discov 7:260 (2021). PubMed: 34552057