AB2230

Anti-BLNK 抗体

Anti-BLNK antibody

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Goat Polyclonal BLNK antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BLNK aa 400 to C-terminus.

別名を表示する

BASH, SLP65, BLNK, B-cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, SLP-65

2 Images

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Western blot - Anti-BLNK antibody (AB2230)

ab2230 staining (4μg/ml) of Daudi lysate (RIPA buffer, 30μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence Primary incubated for 1 hour.

All lanes:

Western blot - Anti-BLNK antibody (ab2230) at 4 µg/mL

All lanes:

Cell lysate prepared from human Daudi cells at 35 µg

Predicted band size: 50 kDa

Observed band size: 70 kDa

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Western blot - Anti-BLNK antibody (AB2230)

All lanes:

Western blot - Anti-BLNK antibody (ab2230) at 1 µg/mL

Lane 1:

Cell lysate prepared from HEK293 mock transfected cells

Lane 2:

Cell lysate prepared from HEK293 overexpressing BLNK

Predicted band size: 50 kDa

Observed band size: 50 kDa

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Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

Synthetic Peptide within Human BLNK aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8WV28

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-4 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. Approx 70kDa band seen in Daudi lysate.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.05% Tris

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.

See full target information BLNK

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BLNK 抗体

Anti-BLNK antibody

Western blot - Anti-BLNK antibody (AB2230)

Western blot - Anti-BLNK antibody (AB2230)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

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Abcam product promise