Anti-BLNK 抗体 (ab15008)
Key features and details
- Rabbit polyclonal to BLNK
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-BLNK antibody
BLNK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BLNK -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment corresponding to BLNK. A fusion protein representing amino acids 171-356 of human BLNK.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Proprietary Purification -
特記事項（精製）
Purified from rabbit antisera by precipitation methods. Purity >95% by SDS-PAGE. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15008の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis. -
組織特異性
Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon. -
関連疾患
Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. -
配列類似性
Contains 1 SH2 domain. -
翻訳後修飾
Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways. -
細胞内局在
Cytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction. - Information by UniProt
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参照データベース
- Entrez Gene: 17060 Mouse
- SwissProt: Q9QUN3 Mouse
- Unigene: 9749 Mouse
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別名
- AGM4 antibody
- B cell adapter containing SH2 domain protein antibody
- B cell adapter containing Src homology 2 domain protein antibody
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab15008 は 7 報の論文で使用されています。
- Zhao G et al. M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties. Cell Death Discov 8:350 (2022). PubMed: 35927251
- Germonpré C et al. Seizures and Interictal Epileptiform Activity in the Rat Collagenase Model for Intracerebral Hemorrhage. Front Neurosci 15:682036 (2021). PubMed: 34220437
- Smith AO et al. A novel strategy for isolation of mice bone marrow endothelial cells (BMECs). Stem Cell Res Ther 12:267 (2021). PubMed: 33941266
- Ding D et al. Spatiotemporal Developmental Upregulation of Prestin Correlates With the Severity and Location of Cyclodextrin-Induced Outer Hair Cell Loss and Hearing Loss. Front Cell Dev Biol 9:643709 (2021). PubMed: 34109172
- Smith AO et al. Isolation of CD31+ Bone Marrow Endothelial Cells (BMECs) from Mice. Bio Protoc 11:e4227 (2021). PubMed: 34909448
- Laue K et al. The maternal to zygotic transition regulates genome-wide heterochromatin establishment in the zebrafish embryo. Nat Commun 10:1551 (2019). PubMed: 30948728
- Rajshekar S et al. Pericentromeric hypomethylation elicits an interferon response in an animal model of ICF syndrome. Elife 7:N/A (2018). PubMed: 30484769