Anti-BLAP75 抗体 (ab70525)
Key features and details
- Rabbit polyclonal to BLAP75
- Suitable for: ICC/IF, IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-BLAP75 antibody -
製品の詳細
Rabbit polyclonal to BLAP75 -
由来種
Rabbit -
特異性
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
アプリケーション
適用あり: ICC/IF, IHC-P, WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
-
免疫原
A region between residues 275 and 300 of human BLAP75 (NP_079221.1)
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70525の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 70 kDa).
|
|
| IP |
Use at 1-4 µg/mg of lysate.
|
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 70 kDa). |
|
IP
Use at 1-4 µg/mg of lysate. |
ターゲット情報
-
機能
Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability. -
配列類似性
Belongs to the RMI1 family. -
細胞内局在
Nucleus. Forms foci in response to DNA damage. - Information by UniProt
-
参照データベース
- Entrez Gene: 80010 Human
- Omim: 610404 Human
- SwissProt: Q9H9A7 Human
- Unigene: 726327 Human
-
別名
- BLAP75 antibody
- BLM associated polypeptide 75 kDa antibody
- BLM associated protein 75 kDa antibody
see all
画像
-
Western blot - Anti-BLAP75 antibody (ab70525)All lanes : Anti-BLAP75 antibody (ab70525) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 70 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 170 kDa, 190 kDa, 238 kDa, 32 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BLAP75 antibody (ab70525)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling BLAP75 with ab70525 at 1/200 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-BLAP75 antibody (ab70525)Detection of Human BLAP75 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70525 at 3 µg/mg for IP and at 1 µg/ml for subsequent WB detection.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab70525 は 3 報の論文で使用されています。
- González-Acosta D et al. PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks. EMBO J 40:e106355 (2021). PubMed: 34128550
- Jia Y et al. Relationship between blood lactic acid, blood procalcitonin, C-reactive protein and neonatal sepsis and corresponding prognostic significance in sick children. Exp Ther Med 14:2189-2193 (2017). PubMed: 28962140
- Ke Y et al. PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution. EMBO J 30:3309-21 (2011). WB ; Human . PubMed: 21743438