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リコンビナントRabMAb

Anti-BIRC6/APOLLON 抗体 [EPR24925-145] (BSA and Azide free) (ab291103)

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Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
  • Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
  • Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
  • Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24925-145] to BIRC6/APOLLON - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-BIRC6/APOLLON antibody [EPR24925-145] (BSA and Azide free)
    BIRC6/APOLLON 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24925-145] to BIRC6/APOLLON - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt (Intra),ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa (human cervix adenocarcinoma epithelial cell) + HeLa treated with 1 µg/mL doxorubicin for 24 hours, 293T (human embryonic kidney epithelial cell), A20 (mouse reticulum sarcoma B lymphocyte), NIH/3T3 (mouse embryonic fibroblast), PC-12 (rat adrenal gland pheochromocytoma), whole cell lysates. IHC-P: Mouse and Rat testis, Mouse liver cancer.

  • 特記事項

    ab291103 is a carrier free version of ab291072

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab291103の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 530 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 530 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF or IP.

ターゲット情報

  • 機能

    Protects cells from undergoing apoptosis.

  • 組織特異性

    Expressed in brain cancer cells.

  • 配列類似性

    In the C-terminal section; belongs to the ubiquitin-conjugating enzyme family.
    Contains 1 BIR repeat.

  • 翻訳後修飾

    Ubiquitinated. Ubiquitination is mediated by the RNF41 E3 ligase and leads to proteasomal degradation, impairing inhibition of apoptosis.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Apollon antibody
      • Apollon protein antibody
      • Baculoviral IAP repeat containing 6 antibody
      • Baculoviral IAP repeat containing protein 6 antibody
      • Baculoviral IAP repeat-containing protein 6 antibody
      • BIR repeat containing ubiquitin conjugating enzyme antibody
      • BIRC 6 antibody
      • BIRC6 antibody
      • BIRC6_HUMAN antibody
      • BRUCE antibody
      • FLJ13726 antibody
      • FLJ13786 antibody
      • KIAA1289 antibody
      • Ubiquitin conjugating BIR domain enzyme apollon antibody
      • Ubiquitin-conjugating BIR domain enzyme apollon antibody
      see all

    画像

    • Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
      Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
      All lanes : Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
      Lane 3 : A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 530 kDa
      Observed band size: 530 kDa


      Exposure time: 48 seconds


      This data was developed using abab291072, the same antibody clone in a different buffer formulation. 

      Blocking / Diluting buffer and concentration:

      5% NFDM/TBST

      Lysates were prepared from fresh materials and used for Western blotting immediately to minimize protein degradation.

    • Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
      Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
      All lanes : Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072) at 1/2000 dilution

      Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
      Lane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 530 kDa
      Observed band size: 530 kDa


      Exposure time: 48 seconds


      This data was developed using abab291072, the same antibody clone in a different buffer formulation. 

      Blocking / Diluting buffer and concentration:

      5% NFDM/TBST

      Lysates were prepared from fresh materials and used for Western blotting immediately to minimize protein degradation.

    • Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
      Western blot - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
      All lanes : Anti-BIRC6/APOLLON antibody [EPR24925-145] (ab291072) at 1/1000 dilution

      Lane 1 : Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HeLa treated with 1 µg/mL doxorubicin for 24 hours, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 530 kDa
      Observed band size: 530 kDa


      Exposure time: 81 seconds


      This data was developed using abab291072, the same antibody clone in a different buffer formulation.

      Blocking / Diluting buffer and concentration:

      5% NFDM/TBST

      The expression level of BIRC6 protein is downregulated in response to doxorubicin treatment (PMID: 23409057). Lysates were prepared from fresh materials and used for Western blotting immediately to minimize protein degradation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)

      This data was developed using abab291072, the same antibody clone in a different buffer formulation. 

      Immunohistochemical analysis of paraffin-embedded mouse testis labeling BIRC6/APOLLON with ab291072 at 1/500 dilution followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Positive Cytoplasmic staining on mouse testis is observed. The section was incubated with ab291072 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)

      This data was developed using abab291072, the same antibody clone in a different buffer formulation. 

      Immunohistochemical analysis of paraffin-embedded rat testis labeling BIRC6/APOLLON with ab291072 at 1/500 dilution followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Positive Cytoplasmic staining on rat testis is observed. The section was incubated with ab291072 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)

      This data was developed using abab291072, the same antibody clone in a different buffer formulation. 

      Immunohistochemical analysis of paraffin-embedded mouse liver cancer labeling BIRC6/APOLLON with ab291072 at 1/500 dilution followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Positive cytoplasmic staining on mouse liver cancer is observed. The section was incubated with ab291072 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    • Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)
      Anti-BIRC6/APOLLON antibody [EPR24925-145] - BSA and Azide free (ab291103)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab291103 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab291103 は論文での使用が確認できていません。

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